Colorimetric and ratiometric fluorescent probe for hydrogen sulfide using a coumarin-pyronine FRET dyad with a large emission shift
Colorimetric and ratiometric fluorescent probe for hydrogen sulfide using a coumarin-pyronine FRET dyad with a large emission shift
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使用具有大发射位移的香豆素-吡喃宁 FRET 二元体检测硫化氢的比色和比率荧光探针
DOI：
10.1039/c6ay02537d
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发表时间：
2016-01-01
影响因子：
3.1
通讯作者：
Lin, Weiying
中科院分区：
文献类型：
--
作者：
He, Longwei;Yang, Xueling;Lin, Weiying
具有两个分辨率良好的发射带的比率荧光探针引起了相当大的关注，因为它们可用于进行比率测量。比率测量可以在一定程度上缓解基于强度的探针因探针浓度效应、探针环境影响和仪器限制而引起的缺点。在此，开发了一种基于香豆素-吡喃宁 FRET 二元体的比色和比率荧光探针 (CP-H2S)，用于体外和体内检测 H2S。在水溶液中，探针表现出通过 FRET 过程从香豆素单元激发的派洛宁单元固有的红色发射，而 CP-H2S 和 H2S 的相遇会抑制 FRET 过程并引发香豆素部分新的蓝色发射，同时红色发射减少。这两个不同发射带的光谱位移高达 119 nm。此外，比率荧光强度信号增强了252.7倍，能量转移效率达到96.7%。这些理想的结果表明 CP-H2S 探头可以显着减少串扰信号并提高比率测量的准确性。在活细胞成像中也获得了 CP-H2S 探针响应 H2S 时的显着信号比率。
Ratiometric fluorescent probes with two well-resolved emission bands have attracted considerable attention, as they can be employed to carry out ratiometric measurements. The ratiometric measurements can alleviate to some extent the shortcomings of intensity-based probes induced by the probe concentration effects, influence of probe environment, and instrumental limitations. Herein, a colorimetric and ratiometric fluorescent probe (CP-H2S) based on a coumarin-pyronine FRET dyad was developed for the detection of H2S in vitro and in vivo. In an aqueous solution, the probe exhibits an inherent red emission of the pyronine unit excited from the coumarin unit by FRET process, whereas an encounter of CP-H2S and H2S suppresses the FRET process and elicits a new blue emission of the coumarin moiety with a concomitant decrease in the red emission. The spectral shift of these two distinct emission bands is up to 119 nm. Moreover, there was a 252.7-fold enhancement in the ratiometric fluorescence intensity signal, and the energy-transfer efficiency was found to be 96.7%. These desirable results suggest that the CP-H2S probe can significantly reduce the crosstalk signals and improve the accuracy of ratiometric measurements. Remarkable ratio of signals of the CP-H2S probe upon responding to H2S was also obtained in living cells imaging.