Monocytes express high amounts of Notch and undergo cytokine specific apoptosis following interaction with the Notch ligand, Delta-1
Monocytes express high amounts of Notch and undergo cytokine specific apoptosis following interaction with the Notch ligand, Delta-1
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DOI：
10.1182/blood.v95.9.2847.009k19_2847_2854
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发表时间：
2000-05-01
期刊：
影响因子：
20.3
通讯作者：
Bernstein, ID
中科院分区：
文献类型：
--
作者：
Ohishi, K;Varnum-Finney, B;Bernstein, ID
Notch信号已被证明在许多发育系统的细胞命运决定中起关键作用。利用逆转录聚合酶链反应（RT-PCR）检测，我们报道了人类Notch-1在CD34+祖细胞中的表达。在这项研究中，我们评估了人Notch-1和Notch-a蛋白在造血细胞中的表达。在免疫荧光研究中，我们在CD34+和CD34+Lin-细胞中检测到少量的Notch-1和Notch-2蛋白，在CD14+单核细胞以及B细胞和T细胞中检测到大量的Notch-1和Notch-2蛋白，但在CD15+粒细胞中未表达。我们进一步发现，Notch配体的固定化截断形式Delta-1在巨噬细胞集落刺激因子（M-CSF）存在时诱导单核细胞凋亡，而不是粒细胞-巨噬细胞集落刺激因子（GMCSF）。Notch蛋白的广泛表达表明该受体在造血过程中具有多种功能。这些研究进一步表明Notch在调节单核细胞存活中的新作用，（Blood. 2000;95:2847-2854） (C) 2000由美国血液学会出版。
Notch signaling has been shown to play a key role in cell fate decisions in numerous developmental systems. Using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay, we reported the expression of human Notch-1 in CD34+ progenitors. In this study, we evaluated the expression of human Notch-1 and Notch-a protein by hematopoietic cells. In immunofluoresence study, we detected low amounts of Notch-1 and Notch-2 protein in both CD34+ and CD34+Lin- cells, high amounts in CD14+ monocytes as well as B and T cells, but no expression in CD15+ granulocytes. We further found that an immobilized truncated form of the Notch ligand, Delta-1, induced apoptosis in monocytes in the presence of macrophage colony-stimulating factor (M-CSF), but not granulocyte-macrophage colony-stimulating factor (GMCSF). The widespread expressions of Notch proteins suggest multiple functions for this receptor during hematopoiesis. These studies further indicate a novel role for Notch in regulating monocyte survival, (Blood. 2000;95:2847-2854) (C) 2000 by The American Society of Hematology.