Relationship between tyrosine phosphorylation and protein expression of insulin receptor and insulin resistance in gestational diabetes mellitus
Relationship between tyrosine phosphorylation and protein expression of insulin receptor and insulin resistance in gestational diabetes mellitus
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妊娠期糖尿病酪氨酸磷酸化与胰岛素受体蛋白表达及胰岛素抵抗的关系
DOI：
10.1007/s11596-014-1289-x
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发表时间：
2014-06-01
影响因子：
--
通讯作者：
Qu, Hong-mei
中科院分区：
文献类型：
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作者：
Chu, Yong-li;Gong, Yu-dian;Qu, Hong-mei
探讨妊娠期糖尿病（GDM）患者酪氨酸磷酸化（TP）与胰岛素受体（InsR）蛋白表达及胰岛素抵抗（IR）的关系。采用Western blotting和免疫沉淀法检测GDM组（GDM组，n=22）、正常妊娠组（正常妊娠组，n=22）和正常非妊娠组（正常非妊娠组，n=13）骨骼肌组织InsR和TP的表达。采用氧化酶法和免疫放射线法测定空腹血糖（FPG）和空腹胰岛素（FINS）。结果显示，GDM组FPG（5.61±0.78 mmol/L）、FINS（15.42±5.13 mU/L）、HOMA-IR（1.21±0.52）水平显著高于正常妊娠组（4.43±0.46 mmol/L、10.56±3.07 mU/L、0.80±0.31）（P<0.01）。正常妊娠组FINS和HOMA-IR水平分别为7.56±2.31 mU/L和0.47±0.26 mU/L，显著高于正常未妊娠组（P<0.01）。三组间InsR表达量差异无统计学意义（P < 0.05）。胰岛素刺激下GDM组InsR TP（0.20±0.05）明显低于正常妊娠组（0.26±0.06）（P<0.01）。正常妊娠组胰岛素刺激下InsR TP低于正常妊娠组（0.31±0.06）（P<0.01）。胰岛素刺激下GDM组InsR TP与HOMA-IR呈负相关（r= - 0.525，P<0.01）。GDM组InsR蛋白表达与HOMA-IR无相关性（r= - 0.236,P < 0.05）。提示GDM骨骼肌InsR蛋白表达与IR无显著相关性，但InsR TP的变化与GDM IR相关。
The relationship between tyrosine phosphorylation (TP) and protein expression of insulin receptor (InsR) and insulin resistance (IR) in patients with gestational diabetes mellitus (GDM) was investigated. The InsR expression and TP in skeleton muscle tissue were determined by Western blotting and immunoprecipitation in women with GDM (GDM group,n=22), normal pregnant women (normal pregnancy group,n=22) and normal non-pregnant women (normal non-pregnant group,n=13). Fasting plasma glucose (FPG) and fasting insulin (FINS) were measured by oxidase assay and immunoradioassay. The results showed that the levels of FPG (5.61±0.78 mmol/L), FINS (15.42±5.13 mU/L) and Homeostasis model assessment-IR (HOMA-IR) (1.21±0.52) in GDM group were significantly higher than those in normal pregnancy group (4.43±0.46 mmol/L, 10.56±3.07 mU/L and 0.80±0.31 respectively) (P<0.01). The levels of FINS and HOMA-IR in normal pregnancy group were significantly higher than those in normal non-pregnant group (7.56±2.31 mU/L and 0.47±0.26 respectively) (P<0.01). There was no significant difference in the InsR expression level among the three groups (P>0.05). TP of InsR with insulin stimulation was significantly decreased in GDM group (0.20±0.05) as compared with normal pregnancy group (0.26±0.06) (P<0.01). TP of InsR with insulin stimulation in normal pregnancy group was lower than that in normal non-pregnant group (0.31±0.06) (P<0.01). TP of InsR with insulin stimulation was negatively related with HOMA-IR in GDM group (r=−0.525,P<0.01). There was no correlation between the protein expression of InsR and HOMA-IR in GDM group (r=−0.236,P>0.05). It was suggested that there is no significant correlation between the protein expression of InsR in skeletal muscle and IR in GDM, but changes in TP of InsR are associated with IR in GDM.