THERMOSTABLE RESTRICTION ENDONUCLEASES FROM THERMOPHILIC BACTERIA
THERMOSTABLE RESTRICTION ENDONUCLEASES FROM THERMOPHILIC BACTERIA
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来自嗜热细菌的耐热限制性内切核酸酶
DOI：
10.7897/2230-8407.050455
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Tanveer Malawat
中科院分区：
文献类型：
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作者：
Gupta Dipali;Sharma Nevadita;Tanveer Malawat
限制性内切酶不仅对细菌和古菌具有重要的防御机制，而且被认为是重组DNA技术的基础。整个生物技术帝国依赖于从种类繁多的细菌中分离出来的II型限制性内切酶。已知的限制性内切酶有4,096种，其中I型106种，II型3种，II型9种，III型21种，IV型19种。其中的6个II型酶已被克隆，611个已测序。从嗜热菌中也分离到了一些限制性内切酶，它们具有较高的热稳定性和对大多数化学变性剂的耐受性。在限制性内切酶数据库中，已经列出了生长温度在400℃以上的约600种II型限制性内切酶。目前，这些酶在分离突变株S和生产反义核酸方面已经发现了重要的作用
Restriction endonucleases are not only important for bacteria and archaea as their defense mechanism but they are c onsidered as foundation of recombinant DNA technology. The whole empire of biotechnology depends upon type II restriction endonucleases isolated from the diversifie d array of bacteria. T here are 4,0 96 known restriction enzymes, including 106 Type I, 3, 9 50 Ty pe II, 21 Type III and 19 Type IV restriction enzymes. Among which 64 6 type II enzymes have been cloned and 611 have been sequenced. A number of restriction endonucleases have been isolated from thermophilic bacteria also, having higher thermal stabilit y and tolerance to most of the chemical denaturants. In Restriction E nzyme Database , about 600 type II restriction enzymes have been listed showing growth temperature above 40 0 C. Nowadays these enzymes have found their importance in isolating mutant strain s and for production of antisense
DOI：
10.1093/protein/13.3.179
发表时间：
2000-03-01
期刊：
PROTEIN ENGINEERING
影响因子：
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作者：
Kumar, S;Tsai, CJ;Nussinov, R
通讯作者：
Nussinov, R
影响因子：
14.9
作者：
POSFAI, J;BHAGWAT, AS;ROBERTS, RJ
通讯作者：
ROBERTS, RJ