INVIVO OXIMETRY USING A NITROXIDE-LIPOSOME SYSTEM
INVIVO OXIMETRY USING A NITROXIDE-LIPOSOME SYSTEM
复制标题
DOI：
10.1002/mrm.1910200113
复制
发表时间：
1991-07-01
影响因子：
3.3
通讯作者：
SWARTZ, HM
中科院分区：
文献类型：
--
作者：
GLOCKNER, JF;CHAN, HC;SWARTZ, HM
含有氘化、带电、水溶性硝基氧 4-三甲基铵-2,2,6,6-四甲基哌啶-d16-1-氧基 (d-Cat1) 的脂质体被用作探针来测量体内氧浓度。肌内或腹膜内注射脂质体悬浮液后，将 ICR 小鼠置于低频 (1.1 GHz) 电子顺磁共振 (EPR) 波谱仪的表面探针上。氘化硝基氧的线宽对溶解氧浓度的变化敏感：该参数单独校准，以便注射小鼠中测量的线宽可以转换为氧张力。随着呼吸气体的氧含量从 21% 变为 85%，再到 0%，该技术检测到 pO2 发生显着变化。静脉注射脂质体也是可能的，脂质体积聚在肝脏和脾脏中，可以测量可检测的氧敏感 EPR 信号。 © 1991 学术出版社
Liposomes containing the deuterated, charged, aqueous soluble nitroxide 4‐trimethylammonium‐2,2,6,6‐tetramethylpiperidine‐d16‐1‐oxyl (d‐Cat1) were used as probes to measure oxygen concentrationsin vivo. Following intramuscular or intraperitoneal injection of the liposome suspension, ICR mice were placed over the surface probe of a low frequency (1.1 GHz) electron paramagnetic resonance (EPR) spectrometer. The linewidth of the deuterated nitroxide is sensitive to changes in the dissolved oxygen concentration: this parameter was calibrated separately so that linewidths measured in the injected mice could be converted into oxygen tensions. This technique detected substantial changes in pO2as the oxygen content of the breathing gas was changed from 21 to 85 to 0%. Intravenous injection of the liposomes also is possible, and the liposomes accumulate in the liver and spleen, where detectable, oxygen‐sensitive EPR signals can be measured. © 1991 Academic Press, Inc.