Gustatory determinants of suckling in albino rats 5-20 days of age.
Gustatory determinants of suckling in albino rats 5-20 days of age.
复制标题
5-20 天龄白化大鼠哺乳的味觉决定因素。
DOI：
10.1002/dev.420180106
复制
发表时间：
1985
影响因子：
2.2
通讯作者：
Blass,EM
中科院分区：
文献类型：
--
作者：
Kehoe,P;Blass,EM
评估了乳鼠幼崽的味觉反应性。实验 I 中的 5 日龄大鼠配备舌管，舌管位于距臼齿前侧 2 mm、尾侧 0-3 mm 或尾侧 4-6 mm 处。哺乳期间以三种方式之一输送水、1.4 × 10−3QHC1、0.50MNH4Cl 或 0.43MNaCl： (1) 连续：液体可从连接到插管的储液器连续获得，并且可以通过施加吸力来获得； (2) 离散：与上述程序相同，只是在 30 分钟测试中每 3 分钟仅提供 30 秒液体； (3) 脉动式：此处，输液泵每 3 分钟 8-10 秒输送 0.05 毫升液体，前提是幼犬附着在乳头上。根据摄入量、时间和行为测量，我们得出结论，特别是对 NH4Cl 和 NaCl 存在行为反应性。至于奎宁，第 5 天的幼犬只有在以连续模式输送 QHCl 时才会有反应。在实验II中，10、15和20天龄的大鼠通过位于三个位置之一的插管输注来接受上述溶液。奎宁反应性从前到后依次发展。 NH4Cl 会扰乱 5、10、尤其是 20 日龄大鼠的行为。值得注意的是，第 15 天的大鼠对 NH4Cl 没有行为反应。氯化钠，特别是在前部和中部位置，在哺乳整个发育过程中被视为令人厌恶的溶液。根据现有的电生理学数据，最后的发现是出乎意料的。
Taste reactivity in suckling rat pups was assessed. Five‐day‐old rats in Experiment I were equipped with tongue cannulae seated 2 mm rostral, 0–3 mm caudal, or 4–6 mm caudal to the intermolar eminence. Either water, 1.4 × 10−3QHC1, .50MNH4Cl, or .43MNaCl was delivered in one of three fashions during suckling: (1) continous: fluid was continuously available from a reservoir connected to the cannula, and could be obtained by exerting suction; (2) discrete: same procedure as above, save that fluid was available for only 30 sec every 3 min of the 30‐min test; (3) pulsatile: here, .05 ml fluid was delivered by infusion pump 8–10 sec every 3 min, provided the pups were attached to a nipple. Based on intake, time and behavioral measures, we conclude behavioral reactivity exists especially to NH4Cl and NaCl. As to quinine, Day 5 pups were responsive only when QHCl was delivered in the continuous mode. In Experiment II, rats 10, 15, and 20 days of age received the above solutions by infusions with the cannulae located in one of the three positions. Quinine reactivity developed sequentially in an anterior to posterior direction. NH4Cl disrupted behavior in 5‐, 10‐, and especilly 20‐day‐old rats. Remarkably, Day 15 rats were not behaviorally reactive to NH4Cl. Sodium chloride, especially in the anterior and middle positions, was treated as an aversive solution during suckling throughout development. Based on existing electrophysiological data, the last finding was unexpected.