"Candidatus phytoplasma australiense" in Coprosma robusta in New Zealand
"Candidatus phytoplasma australiense" in Coprosma robusta in New Zealand
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DOI：
10.1080/0028825x.2004.9512918
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发表时间：
2004-09-01
影响因子：
0.9
通讯作者：
Forster, RLS
中科院分区：
文献类型：
--
作者：
Beever, RE;Wood, GA;Forster, RLS
据报道，植原体来自科普罗斯玛罗布斯塔的野生植物，它与叶子变红或古铜色以及枝条和枝条的枯死有关。它是通过韧皮部组织的电子显微镜以及使用针对 16S rRNA 基因的植原体特异性引物通过一步巢式 PCR 检测到的。 16S产物的序列与Phormium黄叶植原体的序列紧密匹配，并将该植原体置于推定物种“Candidatus Phytoplasma australiense”中。它是通过嫁接到 C.robusta 和 C.macrocarpa 传播的。在克隆匹配的嫁接接种与未嫁接的粗壮种插条中，由植原体（粪菌致死率下降）引起的疾病症状包括脉间失绿和叶子异常变黄或变红、生长减慢和枝条顶枯。对罗布斯塔的实地调查表明，在新西兰大部分地区，枝条枯死和叶色异常很常见。在一些（但不是全部）有症状的植物中检测到“Ca. P. australiense”，表明它可能在田间引起此类症状中发挥作用。据提议，受感染的粗布罗斯塔植物提供了“Ca. P. australiense”的储存库，从而导致其他宿主的感染。
A phytoplasma is reported from wild plants of Coprosma robusta, where it is associated with leaf reddening or bronzing and dieback of shoots and branches. It was detected by electron microscopy of phloem tissue, and by one-step and nested PCR using phytoplasma-specific primers targeting the 16S rRNA gene. The sequence of the 16S product closely matches that of the Phormium yellow leaf phytoplasma and places the phytoplasma in the putative species "Candidatus Phytoplasma australiense". It was transmitted by grafting to C. robusta and C. macrocarpa. The symptoms of the disease caused by the phytoplasma (Coprosma lethal decline) in clonally matched graft-inoculated versus ungrafted cuttings of C. robusta included interveinal chlorosis and abnormal leaf yellowing or reddening, slowing of growth, and shoot dieback. A field survey of C. robusta indicated that shoot dieback and abnormal leaf coloration are common throughout much of New Zealand. "Ca. P. australiense" was detected in some, but not all, symptomatic plants, indicating it may play a role in causing such symptoms in the field. It is proposed that infected C. robusta plants provide a reservoir of "Ca. P. australiense" that leads to infection of other hosts.