ANALYSIS OF 6R-TETRAHYDROBIOPTERIN AND 6S-TETRAHYDROBIOPTERIN AND OTHER PTERINS BY REVERSED-PHASE ION-PAIR LIQUID-CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION BY POSTCOLUMN SODIUM-NITRITE OXIDATION
ANALYSIS OF 6R-TETRAHYDROBIOPTERIN AND 6S-TETRAHYDROBIOPTERIN AND OTHER PTERINS BY REVERSED-PHASE ION-PAIR LIQUID-CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION BY POSTCOLUMN SODIUM-NITRITE OXIDATION
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DOI：
10.1016/0378-4347(93)80495-p
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发表时间：
1993-08-11
期刊：
影响因子：
--
通讯作者：
OHNO, T
中科院分区：
文献类型：
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作者：
TANI, Y;OHNO, T
建立了一种快速灵敏的反相离子对高效液相色谱-柱后亚硝酸钠氧化荧光检测系统，用于6种蝶呤类化合物(6S-5，6，7，8-四氢生物蝶呤、6S-5，6，7，8-四氢生物蝶呤、7，8-二氢生物蝶呤、生物蝶呤、蝶呤和D-新蝶呤)的测定。该方法的变异系数为0.705-3.714%，在信噪比为3的条件下，最小检出量为10-20pg。用上述方法测定了蝶呤类化合物在大鼠组织中的浓度。此外，通过脑微渗析的方法，检测了大鼠纹状体中蝶呤类化合物的产量。因此，这些结果表明，该系统不仅可以用于各种大鼠组织的分析，也可以用于体内收集的透析液的分析。
A rapid and sensitive reversed-phase ion-pair liquid chromatographic system with fluorimetric detection by post-column sodium nitrite oxidation was established for measuring six pterin compounds (6S-5,6,7,8-tetrahydrobiopterin, 6S-5,6,7,8-tetrahydrobiopterin, 7,8-dihydrobiopterin, biopterin, pterin and D-neopterin). The coefficients of variation for these pterins were 0.705-3.714%, and the minimum detectable amount was ca. 10-20 pg at a signal-to-noise ratio of 3. A linear detector response was also verified. The concentrations of the pterin compounds in rat tissues were measured by the described method. Furthermore, by means of brain microdialysis, the output of pterin compounds from rat striatum was detected. Therefore, these results demonstrate that this system can be applied to analyses not only of various rat tissues but also of dialysates collected in vivo.