Regulation of RNA synthesis in yeast III
Regulation of RNA synthesis in yeast III
复制标题
酵母中 RNA 合成的调控 III
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
C. McLaughlin
中科院分区：
文献类型：
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作者：
S. Elliott;C. McLaughlin
摘要离心淘洗法用于从平衡指数生长的酿酒酵母培养物中分离细胞周期不同阶段的细胞。使用脉冲长期标记技术测定DNA和RNA合成速率，该技术能够区分细胞周期中合成速率的指数、线性和周期性变化。研究发现，虽然DNA合成速率在细胞周期中周期性变化，但mRNA、rRNA和tRNA的合成速率在细胞周期中呈指数增长。这些研究结果的影响RNA合成的控制进行了讨论。
SummaryCentrifugal elutriation was used to separate cells in different stages of the cell cycle from a culture of Saccharomyces cerevisiae in balanced exponential growth. The rate of DNA and RNA synthesis was determined using a pulse-long-term label technique that is capable of distinguishing between exponential, linear, and periodic variations in the rate of synthesis through the cell cycle. It was found that while the rate of DNA synthesis varies periodically through the cell cycle, the rate of synthesis of mRNA, rRNA, and tRNA increases exponentially through the cell cycle. The implications of these findings for the control of RNA synthesis are discussed.