Molecular investigation of 2013 dengue Fever outbreak from delhi, India.
Molecular investigation of 2013 dengue Fever outbreak from delhi, India.
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DOI：
10.1371/currents.outbreaks.0411252a8b82aa933f6540abb54a855f
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发表时间：
2014-09-02
期刊：
影响因子：
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通讯作者：
Parveen, Shama
中科院分区：
文献类型：
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作者：
Afreen, Nazia;Deeba, Farah;Parveen, Shama
登革热是一种自限性急性发热性疾病，少数病例可能会加重出血、血浆渗漏和器官损伤。 2013年雨季过后，印度德里爆发了登革热疫情。对疫情爆发期间收集的378份疑似血液样本进行了登革热病毒特异性RT-PCR。 71% 的样本中检测到登革热病毒，其中感染 DENV-2 的患者数量最多（86%），其次是 DENV-1（19%）和 DENV-3（8%）。 14% 的样本中检测到了一种以上 DENV 血清型的双重感染。对 29 个 DENV 毒株（10 个 DENV-1、12 个 DENV-2 和 7 个 DENV-3）进行了部分包膜蛋白基因测序。系统发育分析将 DENV-1 株归为美洲非洲基因型，将 DENV-2 株归为大都会基因型，将 DENV-3 归为基因型 III。我们报告了 2013 年印度德里爆发期间检测到的 29 种 DENV 毒株的血清型分布、循环基因型和部分包膜蛋白基因序列。
Dengue fever is a self-limiting, acute febrile disease which may aggravate to haemorrhage, plasma leakage and organ impairment in small number of cases. An outbreak of dengue fever occurred in Delhi, India after rainy season in the year 2013. Dengue virus specific RT-PCR was carried out on 378 suspected blood samples that were collected during the outbreak. Dengue virus was detected in 71% samples with highest number of patients infected by DENV-2 (86%) followed by DENV-1 (19 %) and DENV-3 (8%). Co-infection with more than one DENV serotype was detected in 14% samples. Twenty nine DENV strains (10 DENV-1, 12 DENV-2 and 7 DENV-3) were sequenced for partial envelope protein gene. Phylogenetic analysis grouped DENV-1 strains in the American African genotype, DENV-2 strains in the Cosmopolitan genotype and DENV-3 in Genotype III. We report the serotype distribution, circulating genotypes and partial envelope protein gene sequence of 29 DENV strains detected during 2013 outbreak in Delhi, India.