Isolation of apoptosis-inducing stilbenoids from four members of the Orchidaceae family.
Isolation of apoptosis-inducing stilbenoids from four members of the Orchidaceae family.
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DOI：
10.1055/s-0031-1280293
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发表时间：
2012
期刊：
影响因子：
2.7
通讯作者：
Russell B Williams;Steven M Martin;Jin-Feng Hu;E. Garo;S. M. Rice;Vanessa L. Norman;Julie A. Lawrence;Grayson W. Hough;M. Goering;M. O’Neil-Johnson;G. Eldridge;C. Starks
中科院分区：
文献类型：
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作者：
Russell B Williams;Steven M Martin;Jin-Feng Hu;E. Garo;S. M. Rice;Vanessa L. Norman;Julie A. Lawrence;Grayson W. Hough;M. Goering;M. O’Neil-Johnson;G. Eldridge;C. Starks
高通量的天然产物研究在兰科的几个物种中产生了一系列抗癌效果（文心兰，O。isthmi和Myrmecophila humboldtii）。还检查了商业文心兰属物种作为附加材料的方便来源。经分离和结构鉴定，共鉴定出15个二苯乙烯类化合物，其中包括一个新的菲醌和两个新的二氢二苯乙烯类化合物。使用Bruker BioSpin TCI 1.7-mm MicroCryoProbe或5-μL CapNMR毛细管微线圈获取用于结构解析和去复制的NMR数据。几种化合物抑制NCI-H460和M14癌细胞系的增殖。还检查了所有化合物诱导细胞凋亡的能力。通过测量NCI-H460细胞系中半胱天冬酶3/7活化和LDH释放来确定凋亡诱导。基于这些结果，对来自市售文心兰属物种的提取物的一部分进行化学改性以试图获得额外的菲醌。
High-throughput natural product research produced a suite of anticancer hits among several species of the Orchidaceae family (Oncidium microchilum, O. isthmi, and Myrmecophila humboldtii). A commercial Oncidium sp. was also examined as a convenient source of additional material. Isolation and structure elucidation led to the identification of fifteen stilbenoids including a new phenanthraquinone and two new dihydrostilbenes. NMR data for structure elucidation and dereplication were acquired utilizing a Bruker BioSpin TCI 1.7-mm MicroCryoProbe or a 5-μL CapNMR capillary microcoil. Several compounds inhibited proliferation of NCI-H460 and M14 cancer cell lines. All compounds were also examined for their ability to induce apoptosis. Apoptosis induction was determined by measuring caspase 3/7 activation and LDH release in a NCI-H460 cell line. Based on these results, a portion of the extract from a commercially available Oncidium sp. was chemically modified in an attempt to obtain additional phenanthraquinones.