Activity-inducible protein Homer1a/Vesl-1S promotes redistribution of postsynaptic protein Homer1c/Vesl-1L in cultured rat hippocampal neurons
Activity-inducible protein Homer1a/Vesl-1S promotes redistribution of postsynaptic protein Homer1c/Vesl-1L in cultured rat hippocampal neurons
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DOI：
10.1016/j.neulet.2003.09.082
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发表时间：
2004-01-09
影响因子：
2.5
通讯作者：
Sugiyama, H
中科院分区：
文献类型：
--
作者：
Inoue, Y;Honkura, N;Sugiyama, H
在培养的大鼠海马神经元中，过表达Homer 1a/Ves 1 - 1 S，诱导蛋白上调癫痫发作或长时程增强，引起突触后蛋白Homer 1c/Ves 1 - 1 L的点状分布减少，而其总量没有显着减少。F-肌动蛋白簇也减少。用BDNF或蛋白酶体抑制剂处理细胞，引起内源性Homer 1a表达水平的增加，也导致Homer 1c斑点的减少。这些结果表明，积累的Homer 1a，无论是外源性表达或内源性诱导，引起重新分配和分散的突触后簇的Homer 1c和F-肌动蛋白，这表明一个重要的作用，Homer 1a在突触重塑。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
In cultured rat hippocampal neurons, overexpression of Homer1a/Ves1-1S, an inducible protein upregulated by seizure or long-term potentiation, caused a reduction of punctate distribution of a postsynaptic protein Homer1c/Ves1-1L, without significant decrease in its total amount. Clusters of F-actin were also decreased. Treatments of cells with BDNF or a proteasome, inhibitor, which cause increase in the expression level of endogenous Homer1a, also resulted in the reduction of Homer1c puncta. These results indicate that the accumulation of Homer1a, either exogenously expressed or endogenously induced, caused redistribution and dispersion of postsynaptic clusters of Homer1c and F-actin, suggesting an important role of Homer1a in synaptic remodeling. (C) 2003 Elsevier Ireland Ltd. All rights reserved.