EFFECT OF CHLORIDES OF MONOVALENT CATIONS, UREA, DETERGENTS, AND HEAT ON MORPHOLOGY AND TURBIDITY OF SUSPENSIONS OF RED HALOPHILIC BACTERIA
EFFECT OF CHLORIDES OF MONOVALENT CATIONS, UREA, DETERGENTS, AND HEAT ON MORPHOLOGY AND TURBIDITY OF SUSPENSIONS OF RED HALOPHILIC BACTERIA
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DOI：
10.1139/m61-088
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发表时间：
1961-01-01
影响因子：
2.8
通讯作者：
GIBBONS, NE
中科院分区：
文献类型：
--
作者：
ABRAM, D;GIBBONS, NE
生长并悬浮在 4.0 [图片] 氯化钠中的红盐杆菌的悬浮液，在转移到浓度逐渐降低的氯化钠时显示出浊度增加，然后突然下降。当将悬浮液置于氯化钾、氯化铷、氯化铯、氯化锂或氯化铵中时，浊度没有增加，但通常随着盐浓度的降低而逐渐降低。在氯化钾、氯化铷和氯化铯中，这些变化与从棒状到过渡形式再到球体的形态变化相关，与氯化钠的变化类似，只是即使在浓度为 4.0 时也发现了过渡形式[图片]。在氯化锂和氯化铵中，分别在 5.0 和 4.5 [图像] 时立即发生变化，并且每根棒形成两到五个小球。在氯化钠和氯化钾的混合物中，细胞的形态取决于钠的离子强度和浓度。还通过将细胞加热至 60-70cC 来产生球体，转化所需的时间随着盐浓度的增加而增加。在尿素溶液中，细胞裂解，但在一定浓度的尿素和氯化钠下观察到细胞变成小球体。离子去污剂导致细胞逐渐崩解。这些观察结果被视为需要钠来维持红色嗜盐棒的细胞壁的进一步证据。
Suspensions of Halobacterium cutirubrum grown and suspended in 4.0 [image] sodium chloride, showed on transfer to increasingly lower concentrations of sodium chloride an increase in turbidity followed by an abrupt decrease. When the suspensions were placed in potassium, rubidium, cesium, lithium, or ammonium chlorides, there was no increase in turbidity but usually a gradual decrease as the concentration of the salt is decreased. In potassium, rubidium, and cesium chlorides these changes were correlated with a change in morphology from rods, through transition forms, to spheres, similar to changes in NaCl, except that transition forms were found even at concentrations of 4.0 [image]. In lithium and ammonium chlorides there was an immediate change at 5.0 and 4.5 [image] respectively and two to five small spheres were formed from each rod. In mixtures of sodium and potassium chlorides the morphology of the cells depended on the ionic strength and concentration of sodium. Spheres were also produced by heating the cells to 60-70cC, the time required for the conversion increasing with increasing salt concentration. In urea solutions the cells lysed, although at certain concentrations of urea and NaCl a change to small spheres was observed. Ionic detergents caused the cells to disintegrate gradually. These observations are taken as further evidence that sodium is required to maintain the cell wall of the red halophilic rods.