Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve
Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve
复制标题
DOI：
10.1016/j.jchemneu.2007.07.001
复制
发表时间：
2008-01-01
影响因子：
2.8
通讯作者：
Villar, Marcelo Jose
中科院分区：
文献类型：
--
作者：
Coronel, Maria Florencia;Brumovsky, Pablo Rodolfo;Villar, Marcelo Jose
单根结扎神经缩窄术（SLNC）是一种新近发展起来的神经病理性疼痛动物模型。大鼠坐骨神经SLNC诱导痛相关行为，以及腰段背根神经节（DRG）和脊髓中神经肽酪氨酸和Y-1受体表达的变化。在本研究中，我们分析了另一种神经肽，甘丙肽，在腰椎背根节和脊髓后，不同程度的限制大鼠坐骨神经的表达。将神经结扎并缩小至其原始直径的10-30、40-80或90%（轻、中或强SLNC）。于伤后7、14、30、60 d取腰4、5背根节及相应节段脊髓，进行甘丙肽免疫组织化学染色。在DR. G，SLNC诱导甘丙肽免疫反应（IR）神经元的数量逐渐增加，与收缩的程度直接相关。因此，在轻度SLNC后，观察到甘丙肽适度上调，主要是在小型神经元中。然而，中等或强SLNC后，甘丙肽-IR神经元的数量有更剧烈的增加，也涉及中型和大型细胞。在损伤后14天检测到最高数量的甘丙肽-IR神经元。在脊髓背角，中等和强SLNCs诱导了显着的同侧增加甘丙肽样免疫反应在板层I-II。这些结果表明甘丙肽在背根节和脊髓中的表达受不同程度的神经收缩的不同调节，并进一步支持其对神经病理性疼痛的调节作用。(c)2007 Elsevier B. V.保留所有权利。
Single ligature nerve constriction (SLNC) is a newly developed animal model for the study of neuropathic pain. SLNC of the rat sciatic nerve induces pain-related behaviors, as well as changes in the expression of neuropeptide tyrosine and the Y-1 receptor in lumbar dorsal root ganglia (DRGs) and spinal cord. In the present study, we have analyzed the expression of another neuropeptide, galanin, in lumbar DRGs and spinal cord after different degrees of constriction of the rat sciatic nerve. The nerve was ligated and reduced to 10-30, 40-80 or 90% of its original diameter (light, medium or strong SLNCs). At different times after injury (7, 14, 30, 60 days), lumbar 4 and 5 DRGs and the corresponding levels of the spinal cord were dissected out and processed for galanin-immunohistochemistry. In DR.Gs, SLNC induced a gradual increase in the number of galanin-immunoreactive (IR) neurons, in direct correlation with the degree of constriction. Thus, after light SLNC, a modest upregulation of galanin was observed, mainly in small-sized neurons. However, following medium or strong SLNCs, there was a more drastic increase in the number of galanin-IR neurons, involving also medium and large-sized cells. The highest numbers of galanin-IR neurons were detected 14 days after injury. In the dorsal horn of the spinal cord, medium and strong SLNCs induced a marked ipsilateral increase in galanin-like immunoreactivity in laminae I-II. These results show that galanin expression in DRGs and spinal cord is differentially regulated by different degrees of nerve constriction and further support its modulatory role on neuropathic pain. (c) 2007 Elsevier B.V. All rights reserved.