POLARIZATION OF YEAST-CELLS IN SPATIAL GRADIENTS OF ALPHA-MATING FACTOR
POLARIZATION OF YEAST-CELLS IN SPATIAL GRADIENTS OF ALPHA-MATING FACTOR
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DOI：
10.1073/pnas.90.18.8332
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发表时间：
1993-09-15
影响因子：
11.1
通讯作者：
SEGALL, JE
中科院分区：
文献类型：
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作者：
SEGALL, JE
酿酒酵母交配过程中的细胞融合过程是由交配伙伴分泌的因子介导的。这些交配因子之一的空间梯度，阿尔法因子，极化了MATA细胞投射的生长。前一次萌发的位置不影响极化方向，去掉交配因子，后一次萌发也发生极化。在诺康唑存在的情况下发生定向，这表明微管不是关键的。在极低的阿尔法因子浓度下，Sst2突变体(在基因研究中不区分产生不同数量的阿尔法因子的伴侣)能够两极分化他们的预测。这种空间感知机制在野生型细胞中的敏感性是这样的，受体占有率的差异估计约为1%，足以用于定位。
The process of cell fusion during mating of the yeast Saccharomyces cerevisiae is mediated hy factors secreted by the mating partners. Spatial gradients of one of these mating factors, alpha-factor, polarized the growth of projections by MATa cells. The site of previous budding did not affect the direction of polarization, and subsequent budding was also polarized if mating factor was removed. Orientation occurred in the presence of nocodazole, suggesting that microtubules were not critical. At extremely low concentrations of alpha-factor, sst2-mutants (which in genetic studies do not discriminate between partners producing different amounts of alpha-factor) were able to polarize their projections. The sensitivity of this spatial sensing mechanism in wild-type cells is such that differences in receptor occupancy estimated to be about 1% are sufficient for orientation.