PAK kinases are directly coupled to the PIX family of nucleotide exchange factors
PAK kinases are directly coupled to the PIX family of nucleotide exchange factors
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DOI：
10.1016/s1097-2765(00)80019-2
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发表时间：
1998-01-01
期刊：
影响因子：
16
通讯作者：
Lim, L
中科院分区：
文献类型：
--
作者：
Manser, E;Loo, TH;Lim, L
PAK激酶家族通过与小GTP酶cdc 42和Rad的相互作用来调节，但对这些蛋白质的上游或下游直接的信号传导组分知之甚少。我们已经纯化并克隆了一类新的Rho-p21鸟嘌呤核苷酸交换因子，通过其N-末端SH 3结构域与保守的富含脯氨酸的PAK序列紧密结合，Kd为24 nM。这种PAK相互作用的交换因子（PIX），这是广泛表达和丰富的Cdc 42和拉德驱动的焦点复合物，是需要PAK招聘到这些网站。PIX可以诱导膜皱褶，并伴随着Rad的激活。我们的研究结果表明，在Cdc 42-到-Rac 1信号转导，涉及PIX/PAK复合物的PIX的作用。
The PAK family of kinases are regulated through interaction with the small GTPases cdc42 and Rad, but little is known of the signaling components immediately upstream or downstream of these proteins. We have purified and cloned a new class of Rho-p21 guanine nucleotide exchange factor binding tightly through its N-terminal SH3 domain to a conserved proline-rich PAK sequence with a K-d of 24 nM. This PAK-interacting exchange factor (PIX), which is widely expressed and enriched in Cdc42- and Rad-driven focal complexes, is required for PAK recruitment to these sites. PIX can induce membrane ruffling, with an associated activation of Rad. Our results suggest a role for PIX in Cdc42-to-Rac1 signaling, involving the PIX/PAK complex.