Cardioprotective effect of betulinic Acid on myocardial ischemia reperfusion injury in rats.
Cardioprotective effect of betulinic Acid on myocardial ischemia reperfusion injury in rats.
复制标题
β酸对大鼠心肌缺血再灌注损伤的心脏保护作用。
DOI：
10.1155/2014/573745
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Zhou W
中科院分区：
文献类型：
--
作者：
Xia A;Xue Z;Li Y;Wang W;Xia J;Wei T;Cao J;Zhou W
目标。本研究旨在探讨白桦酸(BA)对开胸麻醉大鼠心肌缺血再灌注损伤的影响。方法：研究方法。结扎左前降支30min，再灌流2小时。实验分为6组：假手术组、缺血再灌注组、BA低剂量组、BA中剂量组、BA高剂量组、福辛普利钠组。后4组大鼠分别给予BA(50,100,200 mg/kg，i.g)。或福辛普利钠(10 mg/kg，i.g)术前每日1次，连续7天。前两组大鼠给予等量的赋形剂(0.5%CMC-Na，ig)。术中持续监测心功能。用比色法测定血清乳酸脱氢酶(LDH)和肌酸激酶(CK)。免疫印迹法检测心肌细胞Bcl2和Bax的表达，TUNEL法检测心肌细胞的凋亡率。结果。与IR组相比，BA可改善心功能，降低LDH和CK活性。进一步的检测结果表明，Bcl2和Bax的表达与TUNEL法检测结果一致。结论。BA可减少LDH和CK的释放，防止心肌细胞凋亡，最终减轻心肌缺血/再灌注损伤的程度。
Objectives. This study aims to investigate the effect of betulinic acid (BA) on myocardial ischemia reperfusion/injury in an open-chest anesthetized rat model. Methods. The model was induced by 30 minutes left anterior descending occlusion followed by 2 hours reperfusion. There are six groups in our present study: sham operation group, ischemia/reperfusion group, low-dosage BA group, medium-dosage BA group, high-dosage BA group, and fosinopril sodium group. Rats in the latter four groups were administrated with BA (50, 100, and 200 mg/kg, i.g.) or fosinopril sodium (10 mg/kg, i.g.) once a day for 7 days before operation, respectively. Rats in the former two groups were given the same volume of vehicle (0.5% CMC-Na, i.g.). During the operation, cardiac function was continuously monitored. Serum LDH and CK were measured with colorimetric assays. The expression of Bcl-2 and Bax and the apoptosis of cardiomyocytes were investigated with western blot and TUNEL assay, respectively. Results. Pretreatment with BA improved cardiac function and attenuated LDH and CK activities compared with IR group. Further investigation demonstrated that the expression of Bcl-2 and Bax and TUNEL assay was in line with the above results. Conclusion. BA may reduce the release of LDH and CK, prevent cardiomyocytes apoptosis, and eventually alleviate the extent of the myocardial ischemia/reperfusion injury.
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