Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity: Assessment of inflammation in rat and hamster models
Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity: Assessment of inflammation in rat and hamster models
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DOI：
10.1016/0016-5085(84)90202-6
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发表时间：
1984-12
期刊：
影响因子：
29.4
通讯作者：
J. E. Krawisz;P. Sharon;W. Stenson
中科院分区：
文献类型：
--
作者：
J. E. Krawisz;P. Sharon;W. Stenson
设计了一种基于髓过氧化物酶活性评估的定量测定急性肠道炎症的方法。髓过氧化物酶是一种存在于中性粒细胞中的酶，少量存在于单核细胞和巨噬细胞中。髓过氧化物酶用十六烷基三甲基溴化铵溶解，髓过氧化物酶活性用二苯胺- h2o2法测定。在中性粒细胞悬液中，髓过氧化物酶活性与细胞数量直接相关，低至500个细胞。在醋酸性大鼠结肠炎和仓鼠艰难梭菌肠毒素肠炎两种炎症动物模型中测定髓过氧化物酶活性。在两种模型中，从炎症组织中溶解的髓过氧化物酶活性与组织学切片中观察到的中性粒细胞数量成正比。通过在长0.18 mm、厚5 μm的组织学切片上计数中性粒细胞的数量，进行中性粒细胞积累的组织学评估。在这两种动物模型中，髓过氧化物酶活性与400和4000个细胞/mm的中性粒细胞数量呈线性相关。慢性炎症结肠中存在中性粒细胞和组织细胞，其髓过氧化物酶活性与中性粒细胞含量直接相关。组织细胞对髓过氧化物酶活性无显著影响。测定肠道髓过氧化物酶活性是一种简单的生化试验，可用于量化炎症。
An assay was devised to quantitate acute intestinal inflammation based on the assessment of myeloperoxidase activity. Myeloperoxidase is an enzyme found in neutrophils and, in much smaller quantities, in monocytes and macrophages. Myeloperoxidase was solubilized with hexadecyltrimethylammonium bromide and myeloperoxidase activity was measured with a dianisidine-H2O2assay. In neutrophil suspensions, myeloperoxidase activity was directly related to cell number down to as few as 500 cells. Myeloperoxidase activity was assayed in two animal models of inflammation: acetic acid-induced colitis in rats andClostridium difficileenterotoxin-induced enteritis in hamsters. In both models, the activity of myeloperoxidase solubilized from the inflamed tissue was directly proportional to the number of neutrophils seen in histologic sections. Histologic evaluation of neutrophil accumulation was performed by counting the number of neutrophils in a histologic section 0.18 mm long and 5 μm thick. In both animal models, myeloperoxidase activity was linearly related to neutrophil number from 400 and 4000 cells/mm. Myeloperoxidase activity from chronically inflamed colon, in which both neutrophils and histiocytes were present, was directly related to neutrophil content. Histiocytes did not contribute significantly to myeloperoxidase activity. The determination of myeloperoxidase activity in the intestine is a simple biochemical assay that can be used to quantitate inflammation.