Diversity and function of multicopper oxidase genes in the stinkbug Plautia stali
Diversity and function of multicopper oxidase genes in the stinkbug Plautia stali
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DOI：
10.1038/s41598-020-60340-8
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发表时间：
2020-02
影响因子：
4.6
通讯作者：
Yudai Nishide;D. Kageyama;M. Hatakeyama;K. Yokoi;A. Jouraku;Hiromitsu Tanaka;R. Koga;R. Futahashi-R.-Futahas
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作者：
Yudai Nishide;D. Kageyama;M. Hatakeyama;K. Yokoi;A. Jouraku;Hiromitsu Tanaka;R. Koga;R. Futahashi-R.-Futahas
多铜氧化酶（MCO）基因包含细菌、真菌、植物和动物中的多基因家族。 MCO 基因的两个家族 MCO1 (laccase1) 和 MCO2 (laccase2) 在不同昆虫中是保守的，且特征相对较好，而在一些昆虫中也发现了其他 MCO 基因，其生物学功能知之甚少。此前的研究报道MCO1参与肠道免疫，MCO2在昆虫角质层硬化和色素沉着中发挥重要作用。据报道，在蚊子中，MCO2 参与蛋壳硬化和色素沉着，因为 MCO2 的敲低会导致蛋壳变形和脆弱。在这里，我们总共鉴定了7个MCO基因，包括PsMCO1和PsMCO2，并研究了它们在棕翅绿蝽Plautia stali中的表达和功能。通过将双链 RNA (dsRNA) 注射到若虫中，RNA 干扰 (RNAi) 敲低 MCO 基因，结果表明，MCO2（而不是其他 6 个 MCO）是角质层硬化和色素沉着以及 P.P. 生存所必需的。斯塔利。通过将 dsRNA 注射到成年雌性体内（母体 RNAi），跨代敲除 MCO2，导致产生未孵化的蛋，尽管蛋壳没有畸形或脆弱。这些结果表明，MCO2 在角质层的硬化和色素沉着中发挥着重要作用，但在 P 中的蛋壳完整性中不起重要作用。斯塔利。其他 6 个 MCO 基因和 3 个酪氨酸酶基因中任何一个的母体 RNAi 都不影响 P. 的存活率和蛋壳完整性。斯塔利。与在红粉甲虫和糙米飞虱中观察到的相反，MCO6（MCORP；多铜氧化酶相关蛋白）的 RNAi 敲低对稻飞虱没有表现出致死作用。斯塔利。总而言之，我们的研究结果提供了对半翅目和其他昆虫类群 MCO 功能多样性和共性的深入了解。
Multicopper oxidase (MCO) genes comprise multigene families in bacteria, fungi, plants and animals. Two families of MCO genes, MCO1 (laccase1) and MCO2 (laccase2), are conserved among diverse insects and relatively well-characterized, whereas additional MCO genes, whose biological functions have been poorly understood, are also found in some insects. Previous studies reported that MCO1 participates in gut immunity and MCO2 plays important roles in cuticle sclerotization and pigmentation of insects. In mosquitoes, MCO2 was reported to be involved in eggshell sclerotization and pigmentation, on the ground that knockdown of MCO2 caused deformity and fragility of the eggshell. Here we identified a total of 7 MCO genes, includingPsMCO1andPsMCO2, and investigated their expression and function in the brown-winged green stinkbugPlautia stali. RNA interference (RNAi) knockdown of MCO genes by injecting double-stranded RNA (dsRNA) into nymphs revealed that MCO2, but not the other 6 MCOs, is required for cuticle sclerotization and pigmentation, and also for survival ofP. stali. Trans-generational knockdown of MCO2 by injecting dsRNA into adult females (maternal RNAi) resulted in the production of unhatched eggs despite the absence of deformity or fragility of the eggshell. These results suggested that MCO2 plays an important role in sclerotization and pigmentation of the cuticle but not in eggshell integrity inP. stali. Maternal RNAi of any of the other 6 MCO genes and 3 tyrosinase genes affected neither survival nor eggshell integrity ofP. stali. Contrary to the observations in the red flour beetle and the brown rice planthopper, RNAi knockdown of MCO6 (MCORP; Multicopper oxidase related protein) exhibited no lethal effects onP. stali. Taken together, our findings provide insight into the functional diversity and commonality of MCOs across hemipteran and other insect groups.