Multi-step control of spindle pole body duplication by cyclin-dependent kinase
Multi-step control of spindle pole body duplication by cyclin-dependent kinase
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DOI：
10.1038/35050543
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发表时间：
2001-01-01
影响因子：
21.3
通讯作者：
Reed, SI
中科院分区：
文献类型：
--
作者：
Haase, SB;Winey, M;Reed, SI
后生动物中称为中心体的细胞器或酵母中的纺锤体极体（SPB）指导双极纺锤体的组装，这对于有丝分裂期间染色体的忠实分离至关重要。多个中心体的异常积累导致基因组不稳定，并且已经在肿瘤细胞和肿瘤抑制基因中具有靶向突变的细胞中观察到。导致中心体扩增的缺陷尚不清楚。我们通过破坏特定的细胞周期蛋白依赖性激酶（CDK）复合物的活性来重现芽殖酵母中的多中心体表型。我们的观察让人想起的机制，管理DNA复制，并表明，特定的细胞周期蛋白/CDK活动的功能，以促进SPB复制和防止SPB重复。
Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple Centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.