Axonal Gradient of Arachidonic Acid-containing Phosphatidylcholine and Its Dependence on Actin Dynamics*
Axonal Gradient of Arachidonic Acid-containing Phosphatidylcholine and Its Dependence on Actin Dynamics*
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DOI：
10.1074/jbc.m111.316877
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发表时间：
2011-12
期刊：
影响因子：
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通讯作者：
Hyun-Jeong Yang;Y. Sugiura;K. Ikegami;Y. Konishi;M. Setou
中科院分区：
文献类型：
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作者：
Hyun-Jeong Yang;Y. Sugiura;K. Ikegami;Y. Konishi;M. Setou
背景：含花生四烯酸磷脂酰胆碱（AA-PC）的神经元分布尚不清楚。结果：AA-PC轴突强度呈近端-远端梯度，可被肌动蛋白抑制剂破坏。结论：AA-PC在远端轴突中所占比例高于近端轴突，可能与肌动蛋白动力学有关。意义：本研究提供了一个更好地了解PC的神经元的空间组成。磷脂酰胆碱（PC）是脂质双层中最丰富的成分，通过两种酰化脂肪酸的组合以各种分子形式存在。含花生四烯酸（AA）的PC（AA-PC）可以是AA的来源，AA是突触传递和细胞内信号传导的重要介质。然而，AA-PC在神经元内的分布尚未被指出。在本研究中，我们使用成像质谱法来表征PC物种在培养的上级颈神经节神经元中的分布。有趣的是，PC物种表现出独特的分布，这是依赖于在sn-2位的酰基链。特别是，我们发现，AA-PC是丰富的轴突内，分布在近端到远端的梯度。肌动蛋白动力学抑制剂（细胞松弛素D和鬼笔菌素）破坏了这种梯度。这是AA-PC沿着轴突的逐渐分布及其与肌动蛋白动力学的关联的第一份报告。
Background: The neuronal distribution of arachidonic acid-containing phosphatidylcholine (AA-PC) remains unknown. Results: AA-PC axonal intensity showed a proximal-to-distal gradient, which was disrupted by actin inhibitors. Conclusion: AA-PC occupies a higher portion of PC at distal than at proximal axons and may be associated with actin dynamics. Significance: This research provides a better understanding of the neuronal spatial composition of PC. Phosphatidylcholine (PC) is the most abundant component of lipid bilayers and exists in various molecular forms, through combinations of two acylated fatty acids. Arachidonic acid (AA)-containing PC (AA-PC) can be a source of AA, which is a crucial mediator of synaptic transmission and intracellular signaling. However, the distribution of AA-PC within neurons has not been indicated. In the present study, we used imaging mass spectrometry to characterize the distribution of PC species in cultured neurons of superior cervical ganglia. Intriguingly, PC species exhibited a unique distribution that was dependent on the acyl chains at the sn-2 position. In particular, we found that AA-PC is enriched within the axon and is distributed across a proximal-to-distal gradient. Inhibitors of actin dynamics (cytochalasin D and phallacidin) disrupted this gradient. This is the first report of the gradual distribution of AA-PC along the axon and its association with actin dynamics.