Expression profiles of 10,422 genes at early stage of low nitrogen stress in rice assayed using a cDNA microarray
Expression profiles of 10,422 genes at early stage of low nitrogen stress in rice assayed using a cDNA microarray
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DOI：
10.1007/s11103-005-5441-7
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发表时间：
2006-03-01
影响因子：
5.1
通讯作者：
Zhang, QF
中科院分区：
文献类型：
--
作者：
Lian, XM;Wang, SP;Zhang, QF
培育高氮素利用效率的作物品种是农业可持续发展的必然要求。了解植物基因对低氮胁迫的反应对于制定操纵基因以提高NUE的方法至关重要。本研究利用含有11，494个水稻EST（代表10，422个独特基因）的基因芯片，以正常氮为对照，分析了籼稻品种明恢63在苗期低氮胁迫后20 min、1 h和2 h的表达谱。在叶片组织中检测到的差异不显著，而在根组织中检测到的471条ESTs对低N胁迫有响应，其中115条表达上调，358条表达下调。低氮胁迫后的表达谱分析表明：（1）参与光合作用和能量代谢的基因表达迅速下调，（2）许多参与生物和非生物胁迫早期响应的基因表达上调，而许多其他胁迫响应基因表达下调;（3）转录因子和信号转导相关基因表达上调和下调;（4）与氮素吸收和同化有关的基因对低氮胁迫反应不明显。未来研究的挑战是表征低氮胁迫响应基因在氮代谢中的功能作用，包括大量目前功能未知的基因。
Development of crop varieties with high nitrogen use efficiency (NUE) is imperative for sustainable agriculture. Understanding how plant genes respond to low N stress is essential for formulating approaches to manipulating genes for improving NUE. In this study we analyzed the expression profiles of an indica rice cultivar Minghui 63 at seedling stage at 20 min, 1 and 2 h after low N stress with the normal N as the control, using a microarray of 11,494 rice ESTs representing 10,422 unique genes. While no significant difference was detected in the leaf tissue, a total of 471 ESTs were detected as responsive to low N stress in the root tissue with 115 ESTs showing up-regulation and 358 ESTs showing down-regulation. The analysis of expression profiles after low N stress identified following patterns: (1) the genes involved in photosynthesis and energy metabolism were down-regulated rapidly; (2) many of the genes involved in early responses to biotic and abiotic stresses were up-regulated while many other stress responsive genes were down-regulated; (3) regulatory genes including transcription factors and ones involved in signal transduction were both up- and down-regulated; and (4) the genes known to be involved in N uptake and assimilation showed little response to the low N stress. The challenges for future studies are to characterize the functional roles of the low N stress responsive genes in N metabolisms, including the large number of genes presently with unknown functions.