CALCIUM-OXALATE DIHYDRATE FORMATION IN URINE
CALCIUM-OXALATE DIHYDRATE FORMATION IN URINE
复制标题
DOI：
10.1038/ki.1984.115
复制
发表时间：
1984-01-01
影响因子：
19.6
通讯作者：
WERNESS, PG
中科院分区：
文献类型：
--
作者：
MARTIN, X;SMITH, LH;WERNESS, PG
研究了促进尿中草酸钙二水合物（COD）形成的因素。用红外分光光度法检测25毫升等分待测溶液和1毫升0.05 M草酸铵孵育后产生的晶体。利用已知混合物的参考光谱，可以估计COD的分数含量。pH值为6.5时，人类尿液中只会形成COD。在过饱和草酸钙无机溶液中，COD的百分比为7.5 +-。1.4. 焦磷酸盐（1 ~ 8倍）。10-5米)，柠檬酸盐(10-4至2倍。10-3 M)，酵母RNA（5倍）。10-9至0.5倍。10-7 M)，或肝素（2倍）。10-9到2。在过饱和的草酸钙溶液中加入10-7 M)， COD的百分比与添加剂的浓度成正比。硫酸软骨素和Mg对其无影响。pH值的增加增加了含有柠檬酸盐、焦磷酸盐和肝素的无机溶液和未稀释的尿液中COD的形成。rna -柠檬酸盐和柠檬酸盐-焦磷酸盐混合物具有可加性。尿液的效果与其稀释度成反比。在该系统中，促进COD形成的物质在尿中通常存在浓度时具有相稳定作用。这些相同的物质是已知的草酸钙晶体形成的抑制剂。在抑制和相稳定两种情况下，都存在效应的可加性，pH值的变化会改变反应。
Factors that promote the formation of calcium oxalate dihydrate (COD) in urine were investigated. Crystals resulting from the incubation of 25-ml aliquots of the solution to be tested and 1 ml of 0.05 M ammonium oxalate were examined by IR-spectrophotometry. With reference spectra of known mixtures, the fractional content of COD could be estimated. At pH 6.5, only COD formed in human urine. In a supersaturated inorganic solution of calcium oxalate, the percentage of COD was 7.5 .+-. 1.4. Pyrophosphate (1 to 8 .times. 10-5 M), citrate (10-4 to 2 .times. 10-3 M), RNA from yeast (5 .times. 10-9 to 0.5 .times. 10-7 M), or heparin (2 .times. 10-9 to 2 .times. 10-7 M) added to a supersaturated solution of calcium oxalate increased the percentate of COD proportional to the concentration of the additives. Chondroitin sulfate and Mg had no effect. An increase in pH increased the formation of COD in inorganic solutions containing citrate, pyrophosphate, and heparin and in undiluted urine. RNA-citrates and citrate-pyrophosphate mixtures showed additivity. Urine showed an effect that was inversely proportional to its dilution. Substances that promote COD formation in this system had their phase-stabilizing effects at concentrations normally found in urine. These same substances are known inhibitors of calcium oxalate crystal formation. With both inhibition and phase stabilization, there is additivity of effects, and changes in pH alter the response.