A COMPETITIVE-INHIBITION ELISA FOR THE DIFFERENTIATION OF SERUM ANTIBODIES FROM PIGS INFECTED WITH TRANSMISSIBLE GASTROENTERITIS VIRUS (TGEV) OR WITH THE TGEV-RELATED PORCINE RESPIRATORY CORONAVIRUS
A COMPETITIVE-INHIBITION ELISA FOR THE DIFFERENTIATION OF SERUM ANTIBODIES FROM PIGS INFECTED WITH TRANSMISSIBLE GASTROENTERITIS VIRUS (TGEV) OR WITH THE TGEV-RELATED PORCINE RESPIRATORY CORONAVIRUS
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DOI：
10.1016/0378-1135(89)90003-5
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发表时间：
1989-05-01
影响因子：
3.3
通讯作者：
HOOYBERGHS, J
中科院分区：
文献类型：
--
作者：
CALLEBAUT, P;PENSAERT, MB;HOOYBERGHS, J
开发了一种竞争性抑制 ELISA，以单克隆抗体为指示剂，检测猪血清中传染性胃肠炎病毒 (TGEV) 蛋白的非中和抗体。结果表明，TGEV 相关猪呼吸道冠状病毒 (PRCV) 的野生株不会诱导这种抗体，而米勒株和 TGEV 的野生株却能诱导这种抗体。竞争性抑制 ELISA 的敏感性似乎与病毒中和 (VN) 测试相似。该测试能够区分先前感染过 TGEV 或 PRCV 且无法通过经典抗 TGEV 中和测试区分的猪。本测试对于选择性血清诊断是有用的。
A competitive inhibition ELISA was developed to detect non-neutralizing antibodies to the peplomer protein of transmissible gastroenteritis virus (TGEV) in procine sera using a monoclonal antibody as an indicator. It was demonstrated that field strains of the TGEV-related porcine respiratory coronavirus (PRCV) did not induce this antibody, whereas the Miller strain and field strains of TGEV did. The sensitivity of the competitive inhibition ELISA appeared to be similar to that of the virus netralization (VN) test. The test enables differentiation of pigs which were previously infected with TGEV or PRCV and which cannot be distinguished by the classical anti-TGEV neutralization test. The present test is useful for selective serodiagnosis.