High Hydrostatic Pressure-Assisted Enzymatic Hydrolysis Affect Mealworm Allergenic Proteins
High Hydrostatic Pressure-Assisted Enzymatic Hydrolysis Affect Mealworm Allergenic Proteins
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DOI：
10.3390/molecules25112685
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发表时间：
2020-06-01
期刊：
影响因子：
4.6
通讯作者：
Doyen, Alain
中科院分区：
文献类型：
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作者：
Boukil, Abir;Perreault, Veronique;Doyen, Alain
由于世界人口的不断增长，食用昆虫作为替代蛋白质来源引起了越来越多的兴趣。然而，特定昆虫蛋白的致敏性是工业和消费者关注的主要问题。本初步研究考察了高静水压力（HHP）耦合Alcalase（(R)）或胃蛋白酶的酶解能力，以提高粉虫蛋白（特别是过敏原蛋白）的体外消化能力。加压在体外消化前或水解过程中同时进行预处理。比较不同处理之间的水解程度，并采用基于质谱的蛋白质组学方法确定过敏原蛋白的水解效率。只有Alcalase（(R)）在压力下水解提高了粉虫蛋白的水解程度。此外，与胃蛋白酶水解特异性耦合的加压条件增加了主要致敏蛋白的体外消化。因此，hhp辅助酶解代表了一种替代传统水解的策略，用于产生源自过敏原粉虫蛋白的大量肽，并降低其免疫反应性，用于食品，营养保健和制药应用。
Edible insects have garnered increased interest as alternative protein sources due to the world's growing population. However, the allergenicity of specific insect proteins is a major concern for both industry and consumers. This preliminary study investigated the capacity of high hydrostatic pressure (HHP) coupled to enzymatic hydrolysis by Alcalase((R)) or pepsin in order to improve the in vitro digestion of mealworm proteins, specifically allergenic proteins. Pressurization was applied as pretreatment before in vitro digestion or, simultaneously, during hydrolysis. The degree of hydrolysis was compared between the different treatments and a mass spectrometry-based proteomic method was used to determine the efficiency of allergenic protein hydrolysis. Only the Alcalase((R)) hydrolysis under pressure improved the degree of hydrolysis of mealworm proteins. Moreover, the in vitro digestion of the main allergenic proteins was increased by pressurization conditions that were specifically coupled to pepsin hydrolysis. Consequently, HHP-assisted enzymatic hydrolysis represents an alternative strategy to conventional hydrolysis for generating a large amount of peptide originating from allergenic mealworm proteins, and for lowering their immunoreactivity, for food, nutraceutical, and pharmaceutical applications.