Identification of B cell epitopes reactive to human papillomavirus type-16L1- derived peptides.
Identification of B cell epitopes reactive to human papillomavirus type-16L1- derived peptides.
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DOI：
10.1186/1743-422x-9-199
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发表时间：
2012-09-14
期刊：
影响因子：
4.8
通讯作者：
Kamura T
中科院分区：
文献类型：
--
作者：
Fukui A;Matsueda S;Kawano K;Tsuda N;Komatsu N;Shichijo S;Sasada T;Hattori S;Ushijima K;Itoh K;Kamura T
人乳头瘤病毒（HPV）16型和18型的持续感染可导致宫颈癌。为了更好地理解对预防性疫苗HPV 16/18 L1病毒样颗粒（HPV-VLP）的免疫应答，我们研究了HPV 16 L1衍生肽的B细胞表位。分析来自用HPV-16/18 L1 VLP免疫的小鼠的血清的针对10种不同的HPV 16 L1衍生肽（20聚体）的IgG滴度，所述肽含有人白细胞抗原（HLA）-I A-2类，在BalB/c（H-2d）和C57 BL/6（H-2 B）小鼠中，在位置300至319处的一个20-mer肽被鉴定为共同的B细胞表位。作图分析表明，304 - 313位的10个氨基酸序列是免疫原性部分。值得注意的是，该10-mer肽与HLA-A2和HLA-A24分子的结合能力通过HLA I类稳定化测定法证实。此外，一个独特的20聚体被确定为每个菌株中的B细胞表位。这些结果可能为更好地理解HPV 16 L1的免疫反应提供新的信息。
Persistent infection of human papillomavirus (HPV) types 16 and 18 causes cervical cancer. To better understand immune responses to the prophylactic vaccine, HPV 16/18 L1 virus-like particles (HPV-VLPs), we investigated B cell epitopes of HPV16 L1-derived peptides. Sera from mice immunized with HPV-16/18 L1 VLPs were analyzed for their IgG titers against 10 different HPV16 L1-derived peptides (20-mer) that contain human leukocyte antigen (HLA)-class I A-2, A-24 and class II DR. One 20-mer peptide at positions 300 to 319 was identified as a common B cell epitope in both Balb/c (H-2d) and C57BL/6 (H-2b) mice. Mapping analysis showed that the 10-amino-acid sequence at positions 304to 313 was an immunogenic portion. It is of note that the binding capability of this 10-mer peptide to the HLA-A2 and HLA-A24 molecules was confirmed by the HLA class I stabilization assay. In addition, one unique 20-mer was determined as a B cell epitope in each strain. These results might provide new information for better understanding of immune responses to HPV 16 L1.