Usage of the coreceptors CCR-5, CCR-3, and CXCR-4 by primary and cell line-adapted human immunodeficiency virus type 2
Usage of the coreceptors CCR-5, CCR-3, and CXCR-4 by primary and cell line-adapted human immunodeficiency virus type 2
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原代和细胞系适应的 2 型人类免疫缺陷病毒对辅助受体 CCR-5、CCR-3 和 CXCR-4 的使用
DOI：
10.1128/jvi.71.11.8237-8244.1997
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发表时间：
1997
影响因子：
5.4
通讯作者：
M. Alizon
中科院分区：
文献类型：
--
作者：
Nathalie Sol;F. Ferchal;Josephine Braun;O. Pleskoff;C. Tréboute;Isabelle Ansart;M. Alizon
趋化因子受体CCR-5和CXCR-4，可能还有CCR-3，是主要的人类免疫缺陷病毒1型（HIV-1）辅助受体，显然与HIV-1包膜相互作用，与CD 4相关。共表达CD4和这些趋化因子受体的细胞系用一组在外周血单核细胞（PBMC）中传代的7种主要HIV-2分离株和在T细胞系中传代的3种实验室HIV-2株感染。CCR-5、CCR-3和CXCR-4共受体均可被HIV-2利用。使用CXCR-4的能力代表了HIV-2和密切相关的猿免疫缺陷病毒之间的主要差异。大多数使用CCR-5的HIV-2毒株也可以使用CCR-3，有时效率相似。如对HIV-1所观察到的，CCR-5或CCR-3的使用主要见于来自无症状个体的HIV-2毒株，而来自AIDS患者的HIV-2毒株使用CXCR-4。然而，也有一些例外，HIV-2的辅助受体使用模式似乎比HIV-1更复杂。测试的两种T嗜性HIV-2毒株使用CXCR-4而不是CCR-5，而T嗜性HIV-1通常可以使用两者。此外，在5种不能使用CXCR-4的HIV-2毒株中，有3种可以在CCR-5阴性PBMC中复制，这在HIV-1中尚未报道。这些观察结果表明CCR-5辅助受体对HIV-2的重要性低于HIV-1，并表明HIV-2可以使用其他细胞进入途径，可能还有其他辅助受体。一个在正常或CCR-5阴性PBMC中复制的HIV-2分离株未能感染CXCR-4+细胞或U87 MG-CD4和sMAGI细胞系，这些细胞系允许HIV-2感染，但不允许HIV-1感染。这表明存在几种HIV-2特异性辅助受体，它们在细胞系和PBMC中差异表达。
The chemokine receptors CCR-5 and CXCR-4, and possibly CCR-3, are the principal human immunodeficiency virus type 1 (HIV-1) coreceptors, apparently interacting with HIV-1 envelope, in association with CD4. Cell lines coexpressing CD4 and these chemokine receptors were infected with a panel of seven primary HIV-2 isolates passaged in peripheral blood mononuclear cells (PBMC) and three laboratory HIV-2 strains passaged in T-cell lines. The CCR-5, CCR-3, and CXCR-4 coreceptors could all be used by HIV-2. The ability to use CXCR-4 represents a major difference between HIV-2 and the closely related simian immunodeficiency viruses. Most HIV-2 strains using CCR-5 could also use CCR-3, sometimes with similar efficiencies. As observed for HIV-1, the usage of CCR-5 or CCR-3 was observed principally for HIV-2 strains derived from asymptomatic individuals, while HIV-2 strains derived from AIDS patients used CXCR-4. However, there were several exceptions, and the patterns of coreceptor usage seemed more complex for HIV-2 than for HIV-1. The two T-tropic HIV-2 strains tested used CXCR-4 and not CCR-5, while T-tropic HIV-1 can generally use both. Moreover, among five primary HIV-2 strains all unable to use CXCR-4, three could replicate in CCR-5-negative PBMC, which has not been reported for HIV-1. These observations suggest that the CCR-5 coreceptor is less important for HIV-2 than for HIV-1 and indicate that HIV-2 can use other cell entry pathways and probably other coreceptors. One HIV-2 isolate replicating in normal or CCR-5-negative PBMC failed to infect CXCR-4+ cells or the U87MG-CD4 and sMAGI cell lines, which are permissive to infection by HIV-2 but not by HIV-1. This suggests the existence of several HIV-2-specific coreceptors, which are differentially expressed in cell lines and PBMC.
DOI：
10.1073/pnas.94.5.1925
发表时间：
1997-03-04
影响因子：
11.1
作者：
Bleul, CC;Wu, LJ;Mackay, CR
通讯作者：
Mackay, CR