Phosphatidylinositol-hydrolysing enzymes in blowfly salivary glands.
Phosphatidylinositol-hydrolysing enzymes in blowfly salivary glands.
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苍蝇唾液腺中的磷脂酰肌醇水解酶。
DOI：
10.1042/bj2040361
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
R. Dawson
中科院分区：
文献类型：
--
作者：
R. Irvine;M. Berridge;A. Letcher;R. Dawson
成年绿头苍蝇（Calliphora erythrocephala）的唾液腺含有水解磷脂酰肌醇的酶，主要通过不依赖 Ca2+ 的脱酰作用，但也可以检测到 Ca2+ 依赖的磷酸二酯酶（磷脂酶 C）活性。脱酰酶还可以水解磷脂酰胆碱和磷脂酰乙醇胺，并分泌到唾液中。用[3H]肌醇预标记的唾液腺均质化导致内源性3H标记的磷脂酰肌醇快速脱酰化；在匀浆中添加 5-羟色胺不会影响这种水解作用。
The salivary glands of adult blowflies (Calliphora erythrocephala) contain enzymes that hydrolyse phosphatidylinositol, predominantly by a Ca2+-independent deacylation, though a Ca2+-dependent phosphodiesterase (phospholipase C) activity could be detected. The deacylating enzymes could also hydrolyse phosphatidylcholine and phosphatidylethanolamine, and were secreted in the saliva. Homogenization of salivary glands prelabelled with [3H]inositol resulted in a rapid deacylation of the endogenous 3H-labelled phosphatidylinositol; this hydrolysis was unaffected by addition of 5-hydroxytryptamine to the homogenate.