CONTROL OF THE ACTIVITY OF THE SOLUBLE LYTIC TRANSGLYCOSYLASE BY THE STRINGENT RESPONSE IN ESCHERICHIA-COLI
CONTROL OF THE ACTIVITY OF THE SOLUBLE LYTIC TRANSGLYCOSYLASE BY THE STRINGENT RESPONSE IN ESCHERICHIA-COLI
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DOI：
10.1016/0378-1097(90)90187-u
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发表时间：
1990-01-15
影响因子：
2.1
通讯作者：
KECK, W
中科院分区：
文献类型：
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作者：
BETZNER, AS;FERREIRA, LCS;KECK, W
大肠杆菌可溶性溶解性转糖基酶（Slt）是一种体外水解胞壁蛋白的强酶。这里显示它在体内也起自溶素的作用。蛋白质生物合成抑制剂可诱导Slt高产细胞快速自溶，并阻断鸟苷-5 ′-二磷酸-3 ′-二磷酸（ppGpp）的形成。当氨基酸饥饿用于inrelA+而不是inrelA−细胞时。这些发现表明，严格的控制调节可溶性溶解转糖基酶在体内的酶活性。
The soluble lytic transglycosylase (Slt) ofEscherichia coliis known to be a powerful murein hydrolase in vitro. It is shown here to act as an autolysin in vivo as well. Rapid autolysis of Slt overproducing cells was induced by protein bio-synthesis inhibitors, which also block the fomration of guanosine-5′-diphosphate-3′-diphosphate (ppGpp). When amino acid starvation was used to inrelA+but not inrelA−cells. These findings indicate that the stringent control modulates the enzymatic activity of the soluble lytic transglycosylase in vivo.