Comparison of functions of pheromone receptor repertoires in Helicoverpa armigera and Helicoverpa assulta using a Drosophila expression system
Comparison of functions of pheromone receptor repertoires in Helicoverpa armigera and Helicoverpa assulta using a Drosophila expression system
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使用果蝇表达系统比较棉铃虫和棉铃虫信息素受体库的功能
DOI：
10.1016/j.ibmb.2021.103702
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发表时间：
2021-12-27
影响因子：
3.8
通讯作者：
Wang, Chen-Zhu
中科院分区：
文献类型：
--
作者：
Guo, Hao;Huang, Ling-Qiao;Wang, Chen-Zhu
棉蚜和沙蠓是同域近缘种，具有(Z)-11-十六烯醛（Z11-16:Ald）和(Z)-9-十六烯醛（Z9-16:Ald）两种性信息素成分，但比例相反，分别为97:3和3:97。这一特征使它们成为研究鳞翅目昆虫信息素编码机制进化的可行模型。尽管对这两个物种的信息素受体（PR）进行了长达十年的研究，但对这两个物种之间所有PR同源物的功能的比较是不完整的。此外，OR14和OR15的配体到目前为止还没有被发现，可能是由于在化合物面板中缺少活性配体和/或所使用的异源表达系统不相容。在本研究中，我们在果蝇T1神经元中表达了两种Helicoverpa的PR库，以比较研究PR的功能。其中，两种植物的OR13、OR6和OR14都是功能保守且窄调的，表达它们的T1神经元分别对Z11-16:Ald、(Z)9-十六烯醇（Z9-16:OH）和(Z)-11-十六烯乙酸酯（Z11-16:Ac）有反应。表达harmor16的神经元对(Z)-9-十四烯醛（Z9-14:Ald）和(Z)-11-十六烯醇（Z11-16:OH）反应强烈，而表达HassOR16的神经元主要对Z9-14:Ald反应强烈，对(Z)-9-十四烯醇（Z9-14:OH）反应微弱。此外，表达harmor14b的神经元被Z9-14:Ald激活，而表达hassor14b的神经元对Z9-16:Ald、Z9-14:Ald和(Z)-9-十六烯醇（Z9-16:OH）敏感。此外，表达harmor15的神经元对Z9-14:Ald有选择性反应。然而，表达HarmOR11或HassOR11的果蝇T1神经元对所有测试的化合物都沉默。综上所述，我们利用果蝇表达系统和大量的信息素化合物，除OR11外，对这两种Helicoverpa的pr进行了去孤儿化，从而为解析信息素的外显编码提供了有价值的参考。
Helicoverpa armigera and H. assulta are sympatric closely related species sharing two sex pheromone components, (Z)-11-hexadecenal (Z11-16:Ald) and (Z)-9-hexadecenal (Z9-16:Ald) but in opposite ratios, 97:3 and 3:97 respectively. This feature makes them a feasible model for studying the evolution of pheromone coding mechanisms of lepidopteran insects. Despite a decade-long study to deorphanize the pheromone receptor (PR) repertoires of the two species, the comparison of the function of all PR orthologs between the two species is incomplete. Moreover, the ligands of OR14 and OR15 have so far not been found, likely due to the missing of the active ligand(s) in the compound panel and/or incompatibility of heterologous expression systems used. In the present study, we expressed the PR repertoires of both Helicoverpa species in Drosophila T1 neurons to comparatively study the function of PRs. Among those PRs, OR13, OR6, and OR14 of both species are functionally conserved and narrowly tuned, and the T1 neurons expressing each of them respond to Z11-16:Ald, (Z)9-hexadecenol (Z9-16:OH), and (Z)-11-hexadecenyl acetate (Z11-16:Ac), respectively. While HarmOR16expressing neurons respond strongly to (Z)-9-tetradecenal (Z9-14:Ald) and (Z)-11-hexadecenol (Z11-16:OH), the neurons expressing HassOR16 mainly respond to Z9-14:Ald and also weakly respond to (Z)-9-tetradecenol (Z9-14:OH). Moreover, HarmOR14b-expressing neurons are activated by Z9-14:Ald, whereas HassOR14bexpressing neurons are sensitive to Z9-16:Ald, Z9-14:Ald, and (Z)-9-hexadecenol (Z9-16:OH). In addition, HarmOR15-expressing neurons are selectively responsive to Z9-14:Ald. However, the Drosophila T1 neurons expressing either HarmOR11 or HassOR11 are silent to all of the compounds tested. In summary, except for OR11, we have deorphanized all the PRs of these two Helicoverpa species using a Drosophila expression system and a large panel of pheromone compounds, thereby providing a valuable reference for parsing the code of peripheral coding of pheromones.