Real-Time Liquid Crystal pH Sensor for Monitoring Enzymatic Activities of Penicillinase
Real-Time Liquid Crystal pH Sensor for Monitoring Enzymatic Activities of Penicillinase
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DOI：
10.1002/adfm.200900823
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发表时间：
2009-12-09
影响因子：
19
通讯作者：
Yang, Kun-Lin
中科院分区：
文献类型：
--
作者：
Bi, Xinyan;Hartono, Deny;Yang, Kun-Lin
报道了一种基于液晶（LC）的pH传感器，用于实时监测固体表面附近局部pH值的变化，沿着报道了其在酶活性检测中的应用。研究发现，4-氰基-4 ′-戊基联苯（5CB）在掺杂4 ′-戊基联苯-4-羧酸（PBA）后，表现出由亮到暗的光学响应，这可以用PBA在水/液晶界面的质子化和去质子化引起的5CB取向转变来解释.由于其高pH敏感性，基于LC的传感器被进一步开发用于监测由酶促反应引起的局部pH变化。作为概念证明，使用该系统监测表面固定化青霉素酶对青霉素G的水解，即使青霉素G的浓度低至1 nM。这种类型的基于LC的传感器可能会发现潜在的实用程序在高通量筛选酶底物和酶抑制剂。
A liquid crystal (LC)-based pH sensor for real-time monitoring of changes in localized pH values near a solid surface is reported, along with its application for the detection of enzymatic activities. It is found that 4-cyano-4'-pentylbiphenyl (5CB), when doped with 4'-pentyl-biphenyl-4-carboxylic acid (PBA), shows a bright-to-dark optical response can be explained by using orientational transition of 5CB induced by the protonation and deprotonation of PBA at the aqueous/LC interface. Because of its high pH sensitivity, the LC-based sensor is further exploited for monitoring local pH changes resulting from enzymatic reactions. As a proof of concept, the hydrolysis of penicillin G by surface-immobilized penicillinase is monitored using the system, even when the concentration of penicillin G is as low as 1nM. This type of LC-based sensor may find potential utilities in high-throughput screening of enzyme substrates and enzyme inhibitors.