Rate of oxygen consumption by isolated articular chondrocytes is sensitive to medium glucose concentration
Rate of oxygen consumption by isolated articular chondrocytes is sensitive to medium glucose concentration
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DOI：
10.1002/jcp.20491
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发表时间：
2006-02-01
影响因子：
5.6
通讯作者：
Lee, DA
中科院分区：
文献类型：
--
作者：
Heywood, HK;Bader, DL;Lee, DA
软骨组织工程通常涉及在支架材料内培养分离的软骨细胞。已知工程组织内的氧张力是植入成功的基本参数。这将对包埋的软骨细胞的耗氧行为敏感，这仍有待表征。我们报道牛关节软骨细胞的耗氧量对低于2.7 mM的葡萄糖剥夺敏感，从9.6 x 10（-16）mol/cell的基础水平增加到< 18.4 x 10（-16）mol/cell（.）h。进一步的研究检查了选择高（18.4 mM）或低（5.1 mM）葡萄糖培养基对2 mm厚的细胞琼脂糖构建体中氧张力的影响。在低葡萄糖培养基中培养的构建体中观察到耗氧量的相对上调。这导致在接种40 × 10（6）细胞/ml的构建体中接近缺氧的氧浓度为5 μ M，而在相应的高葡萄糖培养物中为57 μ M。氧消耗的上调通常对应于糖酵解的抑制，这与克拉布特里现象一致。中等渗透压（316-600 mOsm）对软骨细胞耗氧率的影响最小。总之，葡萄糖利用率是调节组织工程构建物内氧张力的关键参数。
Cartilage tissue engineering typically involves the culture of isolated chondrocytes within a scaffold material. The oxygen tension within the engineered tissue is known to be an essential parameter for implant success. This will be sensitive to the oxygen consumption behavior of the embedded chondrocytes, which remains to be characterized. We report that the oxygen consumption of bovine articular chondrocytes is sensitive to glucose deprivation below 2.7 mM, increasing from a basal level of 9.6 x 10(-16) to < 18.4 x 10(-16) mol/cell (.) h in 1.3 mM glucose. Further studies examined the influence of selecting high (18.4 mM) or low (5.1 mM) glucose medium on the oxygen tension in 2 mm thick cellular agarose constructs. A relative upregulation of oxygen consumption was observed in constructs cultured in low glucose medium. This resulted in the near-anoxic oxygen concentration of 5 mu M oxygen in constructs seeded with 40 x 10(6) cells/ml, compared to 57 mu M in the corresponding high glucose culture. The upregulation of oxygen consumption generally corresponded to the inhibition of glycolysis, which is consistent with the Crabtree phenomenon. Medium osmolarity (316-600 mOsm) had minimal effects on chondrocyte oxygen consumption rate. In conclusion, glucose availability is a critical parameter that regulates the oxygen tension within tissue engineered constructs.