Highly sensitive detection of nucleocapsid protein from SARS-CoV-2 using a near-infrared surface plasmon resonance sensing system
Highly sensitive detection of nucleocapsid protein from SARS-CoV-2 using a near-infrared surface plasmon resonance sensing system
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DOI：
10.1364/optcon.472486
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发表时间：
2022-11-15
期刊：
影响因子：
--
通讯作者：
Yasui, Takeshi
中科院分区：
文献类型：
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作者：
Kajisa, Taira;Yano, Taka-Aki;Yasui, Takeshi
我们报告了一种近红外表面等离子体共振（SPR）系统，以实现高灵敏度，非标记的检测SARS-CoV-2核衣壳蛋白抗原。近红外光的引入使得SPR角谱的下降变得尖锐，在固定的入射角下，反射光的强度会发生很大的变化。该系统在甘油溶液样品折射率测量中的分辨率达到了10（-5）折射率单位。此外，我们通过将其相应的抗体固定在SPR传感器表面上，测量了SARS-CoV-2的核衣壳蛋白抗原的摩尔浓度为1 fM。该演示表明本系统在医学诊断中用于高灵敏度生物传感的高潜力。(c)2022 Optica出版集团根据Optica开放获取出版协议的条款
We report a near-infrared surface plasmon resonance (SPR) system to achieve highly sensitive, unlabeled detection of the SARS-CoV-2 nucleocapsid protein antigen. Use of the near-infrared light in SPR makes the SPR dip of the angular spectrum sharp and causes a large change of the reflected light intensity at a fixed incident angle. The present SPR system achieves the resolution of 10(-5) refractive index unit in the refractive index measurement of glycerol solution samples. Additionally, we measured the nucleocapsid protein antigen of SARS-CoV-2 down to a molar concentration of 1 fM by immobilizing its corresponding antibody on the SPR sensor surface. This demonstration indicates a high potential of the present system for highly sensitive biosensing in medical diagnostics.(c) 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement