THE CONCENTRATION OF THE MITOCHONDRIAL PYRUVATE CARRIER IN RAT-LIVER AND HEART-MITOCHONDRIA DETERMINED WITH ALPHA-CYANO-BETA-(1-PHENYLINDOL-3-YL)ACRYLATE
THE CONCENTRATION OF THE MITOCHONDRIAL PYRUVATE CARRIER IN RAT-LIVER AND HEART-MITOCHONDRIA DETERMINED WITH ALPHA-CYANO-BETA-(1-PHENYLINDOL-3-YL)ACRYLATE
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DOI：
10.1042/bj2230673
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发表时间：
1984-01-01
影响因子：
4.1
通讯作者：
HALESTRAP, AP
中科院分区：
文献类型：
--
作者：
SHEARMAN, MS;HALESTRAP, AP
.alpha.-Cyano度量。-（1-苯基林多-3-酰基）丙烯酸酯抑制丙酮酸转运到肝脏和心脏线粒体的浓度近似线性，直到达到65%的抑制。抑制程度与线粒体蛋白浓度有关。通过将抑制作用与抑制剂浓度的曲线外推到总抑制作用，或通过对曲线的数学分析，计算出每毫克蛋白质中丙酮酸转运体分子的浓度为。心脏和肝脏线粒体均为100 pmol/mg， Ki约为7 nM。丙酮酸运输在状态3时可能受到心脏线粒体氧化丙酮酸的限制，而不是肝脏线粒体。
.alpha.-Cyano-.beta.-(1-phenylindol-3-yl)acrylate inhibited pyruvate transport into both liver and heart mitochondria approximately linearly with respect to its concentration until 65% inhibition was achieved. The extent of inhibition was dependent on the mitochondrial protein concentration. By extrapolation of plots of inhibition vs. inhibitor concentration to total inhibition, or by mathematical analysis of the plots, the concentration of pyruvate transporter molecules per mg of protein was calculated to be .apprx. 100 pmol/mg for both heart and liver mitochondria, and the Ki about 7 nM. Pyruvate transport is probably rate-limiting for pyruvate oxidation by heart mitochondria in State 3, but not by liver mitochondria.