Basal keratin expression in breast cancer by quantification of mRNA and by immunohistochemistry.
Basal keratin expression in breast cancer by quantification of mRNA and by immunohistochemistry.
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DOI：
10.1186/1756-9966-29-39
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发表时间：
2010-04-28
期刊：
影响因子：
--
通讯作者：
Bednarek A
中科院分区：
文献类型：
--
作者：
Kordek R;Potemski P;Kusinska R;Pluciennik E;Bednarek A
基底样乳腺癌表型的定义各不相同，基于微阵列的表达谱分析仍然是鉴定这些肿瘤的金标准。基底细胞样癌的免疫组化鉴定受到一个事实的阻碍，即在微阵列水平上，并非所有基底细胞样癌都表达基底细胞角蛋白5/6、14和17。我们比较了115例可手术乳腺癌患者中细胞角蛋白5、14和17的表达，采用实时RT-PCR和免疫组织化学方法进行评估。尽管采用了二分法和统计学分析方法，但免疫组化和RT-PCR分析仍有不一致的结果。对于基于四分位数和ROC的二分法，14%的病例在CK 5/6免疫组化检查中为阴性，但呈现高CK 5 mRNA水平。48-55%的病例CK 5/6免疫阳性，但mRNA检测阴性。CK 14和CK 17也观察到类似的不一致性。基底角蛋白mRNA与ER mRNA水平无相关性，而免疫组化与ER状态有显著相关性。我们的观察表明，这两种方法在一小部分情况下可能产生不同的结果。免疫组化和RT-PCR之间的不一致性可能会混淆试图建立一个简单的方法来识别基底细胞样肿瘤。
Definitions of basal-like breast cancer phenotype vary, and microarray-based expression profiling analysis remains the gold standard for the identification of these tumors. Immunohistochemical identification of basal-like carcinomas is hindered with a fact, that on microarray level not all of them express basal-type cytokeratin 5/6, 14 and 17. We compared expression of cytokeratin 5, 14 and 17 in 115 patients with operable breast cancer estimated by real-time RT-PCR and immunohistochemistry. Despite the method of dichotomization and statistical analysis, there were cases with discordant results comparing immunohistochemistry and RT-PCR analysis. For dichotomisation based on quartiles and ROC, 14% of cases were negative on immunohistochemical examination for CK5/6, but presented high CK5 mRNA levels. There were also 48-55% cases, which were CK5/6-immunopositive, but were negative by mRNA examination. Similar discordances were observed for CK14 and CK17. Basal keratin mRNAs did not correlate with ER mRNA levels, while immunohistochemistry produced significant relationship with ER status. Our observation suggest that both method may produce different results in a small proportion of cases. Discordance between immunohistochemistry and RT-PCR may confound attempts to establish a simple methods for identification of basal-like tumors.
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DOI：
10.1186/bcr1636
发表时间：
2007
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Fulford LG;Reis-Filho JS;Ryder K;Jones C;Gillett CE;Hanby A;Easton D;Lakhani SR
通讯作者：
Lakhani SR
基础样的表型与雌激素受体阴性乳腺癌中的患者存活无关。
影响因子：
7.4
作者：
Jumppanen, Mervi;Gruvberger-Saal, Sofia;Kauraniemi, Paivikki;Tanner, Minna;Bendahl, Par-Ola;Lundin, Mikael;Krogh, Morten;Kataja, Pasi;Borg, Ake;Ferno, Marten;Isola, Jorma
通讯作者：
Isola, Jorma
DOI：
10.1073/pnas.96.16.9212
发表时间：
1999-08-03
影响因子：
11.1
作者：
Perou, CM;Jeffrey, SS;Botstein, D
通讯作者：
Botstein, D
影响因子：
3.8
作者：
Byrski, T.;Huzarski, T.;Narod, S. A.
通讯作者：
Narod, S. A.
免疫组织化学标记物的使用可以完善三乳腺癌的预后。
影响因子：
3.8
作者：
Tischkowitz, Marc;Brunet, Jean-Sebastien;Begin, Louis R.;Huntsman, David G.;Cheang, Maggie Cu;Akslen, Lars A.;Nielsen, Torsten O.;Foulkes, William D.
通讯作者：
Foulkes, William D.