Molecular basis of sperm flagellar axonemes - Structural and evolutionary aspects
Molecular basis of sperm flagellar axonemes - Structural and evolutionary aspects
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DOI：
10.1196/annals.1389.017
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Inaba, Kazuo
中科院分区：
文献类型：
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作者：
Inaba, Kazuo
轴突体在精子鞭毛中起运动机械的作用。虽然在某些情况下观察到非典型轴突结构，但在许多生物体中轴突的9 + 2微管结构占主导地位。一些结构与这些微管结合，组成一个高度组织化的蛋白质网络。轴突蛋白的广泛蛋白质组学分析导致在轴突蛋白中发现了几个重复序列、结构域和基序。对海鞘和绿藻类莱茵衣藻轴突亚结构亚基组成的分子比较，揭示了细胞内功能复合体进化的一个有趣的分子方面：轴突的结构在进化过程中得到了很好的保守，但每个轴突组成部分的分子结构并不总是保守的。从轴突蛋白的结构域结构来看，外臂动力蛋白和径向辐条等亚结构包含一组结构域结构，尽管这两种生物的某些结构域亚基不同。因此，亚结构中蛋白质结构域的保护似乎优先于每个蛋白质的保护（“模块优势保护”），这可能最终导致轴突染色体通过进化在形态和功能上的保护。
The axonemes serve as motile machineries in sperm flagella. Although atypical axonemal structures are observed in some cases, 9 + 2 microtubule structure of the axoneme is predominant in many organisms. Several structures are bound to these microtubules and comprise a highly organized protein network. Extensive proteomic analysis of the axonemes has led to find several repeats, domains, and motifs in axonemal proteins. Molecular comparison of subunit composition of axonemal substructures between the ascidian Ciona intestinalis and the green algae Chlamydomonas reinhardtti leads to an intriguing molecular aspect concerning the evolution of intracellular functional complex: The architecture of the axonemes has been well conserved through evolution, but the molecular structure of each axonemal component is not always conserved. In light of domain structure in the axonemal proteins, substructures like outer arm dynein and radial spoke contain a set of domain structures, although some domain-containing subunits are different between these two organisms. Thus, conservation of protein domains within a substructure seems to take precedence over that of each protein ("module-dominant conservation"), which may ultimately result in morphological and functional conservation of the axonemes through evolution.