Neuroimmune regulation of colonic secretion in guinea pigs.
Neuroimmune regulation of colonic secretion in guinea pigs.
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DOI：
10.1152/ajpgi.1991.260.2.g307
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发表时间：
1991-02
期刊：
影响因子：
--
通讯作者：
Y. -. Wang;J. Palmer;H. Cooke
中科院分区：
文献类型：
--
作者：
Y. -. Wang;J. Palmer;H. Cooke
用2×10(3)感染旋毛虫幼虫免疫豚鼠6~8wk，观察黏膜下神经元在结肠上皮过敏样反应中的作用。血清中加入旋毛虫抗原(20微克/毫升)到建立在流量室中的肌肉剥离的结肠节段，在免疫的豚鼠(但不是非免疫的)豚鼠中，在5分钟内引起短路电流的最大增加。膜稳定剂槲皮素和组胺H1受体拮抗剂吡拉明可减弱旋毛虫抗原引起的上皮反应。河豚毒素和M受体拮抗剂阿托品可降低抗原诱导的上皮细胞反应，但不能通过甲基胺阻断烟碱受体而减少。免疫豚鼠结肠黏膜的抗原攻击增强了对电场刺激和P物质引起的内源性神经递质的分泌反应。在有抗原存在的情况下，河豚毒素不敏感的卡巴胆碱反应被增强，并被槲皮素逆转，但不能被吡拉明逆转。结果提示，黏膜下胆碱能神经在旋毛虫免疫豚鼠结肠黏膜对虫体抗原诱导的快速上皮反应中起重要作用。免疫介质与黏膜下神经丛中神经递质的相互作用增强了旋毛虫在免疫宿主中触发的分泌性黏膜反应。
The role of submucosal neurons in anaphylactic-like responses in colonic epithelium from immunized guinea pigs was examined 6-8 wk after inoculation with 2 x 10(3) infective Trichinella spiralis larvae. Serosal addition of T. spiralis antigen (20 micrograms/ml) to muscle-stripped segments of colon set up in flux chambers evoked a maximum increase in short-circuit current within 5 min in immune, but not nonimmune, guinea pigs. Quercetin, a membrane-stabilizing drug, and pyrilamine, a histamine H1 receptor antagonist, attenuated epithelial responses evoked by T. spiralis antigen. Antigen-induced epithelial responses were reduced by neural blockade with tetrodotoxin and by the muscarinic receptor antagonist atropine but not by blockade of nicotinic receptors with mecamylamine. Antigenic challenge of colonic mucosa from immune guinea pigs enhanced the secretory responses to endogenously released neurotransmitters evoked by electrical field stimulation and substance P. In the presence of antigen, the tetrodotoxin-insensitive component of the carbachol response was enhanced and was reversed by quercetin but not pyrilamine. The results suggest that submucosal cholinergic nerves play a role in mediating the rapid epithelial responses evoked by worm antigen in the colonic mucosa of T. spiralis-immune guinea pigs. Interaction of immunological mediators with neurotransmitters in the submucosal plexus augments the secretory mucosal response triggered by T. spiralis in immunized hosts.