Evidence that the fertilization envelope blocks sperm entry in eggs of Xenopus laevis: interaction of sperm with isolated envelopes.
Evidence that the fertilization envelope blocks sperm entry in eggs of Xenopus laevis: interaction of sperm with isolated envelopes.
复制标题
受精包膜阻止精子进入非洲爪蟾卵的证据：精子与孤立包膜的相互作用。
DOI：
10.1016/0012-1606(76)90285-2
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发表时间：
1976
影响因子：
2.7
通讯作者：
Jerry L. Hedrick
中科院分区：
文献类型：
--
作者：
Robert D. Grey;Jerry L. Hedrick
非洲爪蟾卵的皮层反应导致卵黄被膜（VE）向受精被膜（FE）的转化。FE是由前VE加上一个新的组成部分，受精层，形成作为皮质反应的结果。建立了体外精子穿透力测定系统，比较了VE和FE对精子穿透力的影响。VE和FE分别从未受精卵和卵裂胚胎中分离。虽然它们在分离过程中膨胀，但分离的VE和FE保留了它们可以原位固定的形态特征。通过将包膜悬浮液暴露于盐水溶液中的精子来测试分离包膜的渗透性，所述盐水溶液中加入了来自卵的外膜的溶解组分。分离的VE被来自两侧的精子穿透。相比之下，孤立的FE被证明是不可穿透的：在任何情况下，精子都没有穿透受精层或FE的VE成分。我们的结论是，受精包膜阻止精子进入非洲爪蟾。
The cortical reaction in eggs ofXenopus laevisresults in conversion of the vitelline envelope (VE) into the fertilization envelope (FE). The FE is composed of the former VE plus a new component, the fertilization layer, that forms as a consequence of the cortical reaction. Anin vitrotest system was developed to compare the VE and FE in terms of their penetrability by sperm. VE's and FE's were isolated from unfertilized eggs and cleaving embryos, respectively. Although they swell during the isolation procedure, the isolated VE's and FE's retain the morphological characteristics by which they can be distinguishedin situ. Penetrability of isolated envelopes was tested by exposing suspensions of envelopes to sperm in a saline solution to which solubilized components from the jellycoat of the egg were added. Isolated VE's were penetrated by sperm from both sides. By contrast, isolated FE's proved impenetrable: In no instance did sperm penetrate either the fertilization layer or the VE component of the FE. We concluded that the fertilization envelope blocks sperm entry inXenopus laevis.