BACILLUS-THURINGIENSIS TOXIN INHIBITS K+-GRADIENT-DEPENDENT AMINO-ACID-TRANSPORT ACROSS THE BRUSH-BORDER MEMBRANE OF PIERIS-BRASSICAE MIDGUT CELLS
BACILLUS-THURINGIENSIS TOXIN INHIBITS K+-GRADIENT-DEPENDENT AMINO-ACID-TRANSPORT ACROSS THE BRUSH-BORDER MEMBRANE OF PIERIS-BRASSICAE MIDGUT CELLS
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DOI：
10.1016/0014-5793(86)80814-6
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发表时间：
1986-08-18
期刊：
影响因子：
3.5
通讯作者：
WOLFERSBERGER, MG
中科院分区：
文献类型：
--
作者：
SACCHI, VF;PARENTI, P;WOLFERSBERGER, MG
幼虫中肠细胞的腔膜是苏云金芽孢杆菌杀虫三角洲内毒素的作用部位。在与体内正常有效剂量相对应的浓度下，该毒素可抑制从青花pieris brassicaailva中部制备的刷状边缘膜囊泡对氨基酸的吸收。毒素不与K+-氨基酸同调相互作用，而是增加膜的K+通透性。毒素不会增加鳞翅目中肠刷边膜对Na+或H+的通透性，也不会增加哺乳动物小肠制备的刷边膜小泡对K+的通透性。
The luminal membrane of larval midgut cells is the site of action of insecticidal delta-endotoxin fromBacillus thuringiensis. At concentrations that correspond to normal effective doses in vivo, the toxin inhibits the uptake of amino acids by brush border membrane vesicles prepared from midguts ofPieris brassicaelarvae. The toxin does not interact with the K+-amino acid symport but rather increases the K+permeability of the membrane. The toxin does not increase the permeability of lepidopteran midgut brush border membrane to either Na+or H+nor does it increase the K+permeability of brush border membrane vesicles prepared from mammalian small intestine,