Genome-wide localization analysis of a complete set of Tafs reveals a specific effect of the taf1 mutation on Taf2 occupancy and provides indirect evidence for different TFIID conformations at different promoters
Genome-wide localization analysis of a complete set of Tafs reveals a specific effect of the taf1 mutation on Taf2 occupancy and provides indirect evidence for different TFIID conformations at different promoters
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DOI：
10.1093/nar/gkp1172
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发表时间：
2010-04-01
影响因子：
14.9
通讯作者：
Kokubo, Tetsuro
中科院分区：
文献类型：
--
作者：
Ohtsuki, Kazushige;Kasahara, Koji;Kokubo, Tetsuro
在酿酒酵母中，TFIID和佐贺主要通过将TBP递送到核心启动子来分别介导组成型管家基因和胁迫诱导基因的转录。两者都是分别由15个和20个亚基组成的多蛋白质复合物，其中5个亚基是共同的，并且可能构成每个复合物中的核心子模块。虽然全基因组基因表达的研究已经进行了广泛的几个TFIID和/或佐贺突变体，只有有限数量的研究调查这两个复合物的成分的全基因组定位。具体地，没有关于完整的一组Tafs的定位和taf突变对定位的影响的先前报道。在这里，我们通过染色质免疫沉淀（ChIP）-芯片分析野生型和taf 1-T657 K突变株，检查了一整套Tafs、Gcn 5、Bur 6/Ncb 2、Sua 7、Tfa 2、Tfg 1、Tfb 3和Rpb 1在染色体III、IV和V上的定位谱。此外，我们进行了常规和连续的ChIP分析的几个核糖体蛋白基因（RPGs）和非RPGs。有趣的是，这些结果揭示了TFIIB和NC 2之间的新关系，佐贺和TFIID在RPG启动子上的同时共定位，taf 1突变对Taf 2占用的特定影响，以及不同TFIID构象存在的间接证据。
In Saccharomyces cerevisiae, TFIID and SAGA principally mediate transcription of constitutive housekeeping genes and stress-inducible genes, respectively, by delivering TBP to the core promoter. Both are multi-protein complexes composed of 15 and 20 subunits, respectively, five of which are common and which may constitute a core sub-module in each complex. Although genome-wide gene expression studies have been conducted extensively in several TFIID and/or SAGA mutants, there are only a limited number of studies investigating genome-wide localization of the components of these two complexes. Specifically, there are no previous reports on localization of a complete set of Tafs and the effects of taf mutations on localization. Here, we examine the localization profiles of a complete set of Tafs, Gcn5, Bur6/Ncb2, Sua7, Tfa2, Tfg1, Tfb3 and Rpb1, on chromosomes III, IV and V by chromatin immunoprecipitation (ChIP)-chip analysis in wild-type and taf1-T657K mutant strains. In addition, we conducted conventional and sequential ChIP analysis of several ribosomal protein genes (RPGs) and non-RPGs. Intriguingly, the results revealed a novel relationship between TFIIB and NC2, simultaneous co-localization of SAGA and TFIID on RPG promoters, specific effects of taf1 mutation on Taf2 occupancy, and an indirect evidence for the existence of different TFIID conformations.