Different chemokine expression in lethal and non-lethal murine West Nile virus infection
Different chemokine expression in lethal and non-lethal murine West Nile virus infection
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DOI：
10.1002/jmv.20205
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发表时间：
2004-11-01
影响因子：
12.7
通讯作者：
Takashima, I
中科院分区：
文献类型：
--
作者：
Shirato, K;Kimura, T;Takashima, I
西尼罗河病毒（WN）是一种蚊媒黄病毒，可导致人类和马致命的脑炎。1999年在纽约市（NY）流行的WN病毒引起了野生鸟类的大规模死亡，这在世界其他地区的流行区并不明显，在纽约分离的WN病毒株（NY株）的发病机制似乎与以前分离的毒株不同。然而，NY株感染的发病机制仍不清楚。本研究检测了小鼠感染致死性NY株和非致死性Eg 101株期间CC（RANTES/CCL 5、MIP-1 α/CCL 3、MIP-1 β/CCL 4）和CXC（IP-10/CXCL 10、B淋巴细胞趋化因子（BLC/CXCL 13）和B细胞和单核细胞活化趋化因子（BMAC/CXCL 14））趋化因子的表达。我们发现CC趋化因子RANTES、MIP-1 α、MIP-1 β和IP-10的mRNA在NY株感染小鼠的脑中高度上调。相比之下，在两组小鼠中均未检测到BLC mRNA，并且BMAC mRNA在非致死性Eg 101菌株感染的晚期阶段相对于NY菌株感染的小鼠中的水平高度上调。(C)2004 Wiley-Liss，Inc.
West Nile (WN) virus is a mosquito-borne flavivirus that can cause lethal encephalitis in humans and horses. The WN virus endemic in New York City (NY) in 1999 caused large-scale mortality of wild birds that was not evident in endemic areas in other parts of the world, and the pathogenesis of the WN virus strain isolated in NY (NY strain) appears to differ from that of previously isolated strains. However, the pathogenesis of NY strain infection remains unclear. This study examined CC (RANTES/CCL5, MIP-1alpha/CCL3, MIP-1beta/CCL4) and CXC (IP-10/CXCL10, B lymphocyte chemoattractant (BLC/CXCL13), and B cell- and monocyte-activating chemokine (BMAC/CXCL14)) chemokine expression during lethal NY strain and non-lethal Eg101 strain infection in mice. We found that the mRNA of the CC chemokines, RANTES, MIP-1alpha, MIP-1beta, and IP-10 was highly up-regulated in the brain of NY strain-infected mice. By contrast, BLC mRNA was not detected in either group of mice, and BMAC mRNA was highly up-regulated in late stage of infection with the non-lethal Eg101 strain relative to levels in NY strain-infected mice. (C) 2004 Wiley-Liss, Inc.