Induction of Apoptosis in Human Papillary-Thyroid-Carcinoma BCPAP Cells by Diallyl Trisulfide through Activation of the MAPK Signaling Pathway
Induction of Apoptosis in Human Papillary-Thyroid-Carcinoma BCPAP Cells by Diallyl Trisulfide through Activation of the MAPK Signaling Pathway
复制标题
二烯丙基三硫化物通过激活 MAPK 信号通路诱导人乳头状甲状腺癌 BCPAP 细胞凋亡
DOI：
10.1021/acs.jafc.8b02243
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发表时间：
2018-06-13
影响因子：
6.1
通讯作者：
Lu, Rongrong
中科院分区：
文献类型：
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作者：
Pan, Jie;Zhang, Li;Lu, Rongrong
本研究旨在探讨二烯丙基三硫（DATS）对人甲状腺乳头状癌BCPAP细胞的作用及其机制。DATS是一种来自大蒜的有机硫化合物。在这项研究中，我们证明，与溶剂对照相比，浓度为5，10和20 μ M的DATS处理使BCPAP细胞的细胞存活率分别降低至84.51 +/- 2.67，57.16 +/- 1.18和41.22 +/- 1.19%。DATS还使BCPAP细胞周期阻滞于G 0/G1期，阻滞于G 0/G1期的细胞比例从68.8 ± 8.38%上升到80.4 ± 8.38%，最终通过线粒体凋亡途径导致细胞凋亡。进一步的证据表明，DATS激活ERK，JNK和p38，MAPK家族的成员。ERK和JNK抑制剂可部分逆转DATS诱导的BCPAP细胞凋亡。综上所述，我们的研究结果表明，DATS通过激活MAPK信号通路对甲状腺乳头状癌细胞产生凋亡诱导作用，这为甲状腺癌治疗提供了一个有前景的治疗靶点。
This study aimed to elucidate the potential effects of diallyl trisulfide (DATS) on human papillary-thyroid carcinoma BCPAP cells and its underlying mechanisms. DATS is an organosulfur compound derived from garlic. In this study, we demonstrated that compared with the solvent control, DATS treatment at concentrations of 5, 10, and 20 mu M decreased cell survival rates of BCPAP cells to 84.51 +/- 2.67, 57.16 +/- 1.18, and 41.22 +/- 1.19% respectively. DATS also caused cell-cycle arrest at G0/G1 phase, and the proportion of cells arrested in G0/G1 phase rose from 68.8 +/- 8.38 to 80.4 +/- 8.38%, which eventually resulted in cell apoptosis through a mitochondrial apoptotic pathway in BCPAP cells. Further evidence showed that DATS activated ERK, JNK, and p38, members of the MAPK family. Moreover, ERK and JNK inhibitors partially reversed apoptosis in BCPAP cells induced by DATS treatment. Taken together, our results demonstrated that DATS exerted an apoptosis-inducing effect on papillary-thyroid-cancer cells via activation of the MAPK signaling pathway, which shed light on a prospective therapeutic target for thyroid-cancer treatment.