Antioxidant responses to simulated acid rain and heavy metal deposition in birch seedlings
Antioxidant responses to simulated acid rain and heavy metal deposition in birch seedlings
复制标题
DOI：
10.1016/s0269-7491(96)00071-1
复制
发表时间：
1997-01-01
影响因子：
8.9
通讯作者：
Hanninen, O
中科院分区：
文献类型：
--
作者：
Koricheva, J;Roy, S;Hanninen, O
本研究测量了 2 年生桦树 (Betula pendula Roth) 幼苗在模拟酸雨 (pH 4.0) 和重金属 (Cu/Ni) 下的反应，单独或联合施用 2 个月。污染物的施用浓度没有显着影响幼苗生物量或总谷胱甘肽水平。单独的酸雨会增加叶子和根中的超氧化物歧化酶（SOD）活性，而单独的重金属会抑制根中的 SOD 活性。酸雨和重金属单独施用都会增加叶片中抗坏血酸过氧化物酶（APX）和愈创木酚过氧化物酶（GPX）的活性，但会降低根部的活性。相比之下，酸雨和重金属处理会增加根部的谷胱甘肽还原酶（GR）活性，但不会增加叶子的活性。用酸雨和重金属的混合物喷洒桦树幼苗可以增加叶子中的 SOD、APX 和 GPX 活性以及根中的 GR 活性。然而，混合污染物对酶活性的影响通常小于单个污染物影响的总和。酶的反应还取决于污染物的施用位置：将污染物喷洒到枝条上比施用于土壤表面会引发更高的 SOD、APX 和 GPX 反应，而 GR 则相反。 (C) 1997 Elsevier Science Ltd. 保留所有权利。
This study measured the responses of different antioxidants in 2-year-old birch (Betula pendula Roth) seedlings subjected to simulated acid rain (pH 4.0) and heavy metals (Cu/Ni), applied alone or in combination for 2 months. The applied concentrations of pollutants did not significantly affect seedling biomass or total glutathione levels. Acid rain alone increased superoxide dismutase (SOD) activity both in leaves and roots, while heavy metals alone inhibited SOD activity in roots. Both acid rain and heavy metals applied singly increased ascorbate peroxidase (APX) and guaiacol peroxidase (GPX) activities in leaves but decreased activities in roots. In contrast, acid rain and heavy metal treatments increased glutathione reductase (GR) activity in roots but not in leaves. Spraying birch seedlings with a mixture of acid rain and heavy metals increased SOD, APX and GPX activities in leaves and GR activity in roots. However, the effects of mixed pollutants on enzyme activities usually were less than the summed effects of individual pollutants. Enzyme responses also depended on where pollutants were applied: spraying pollutants onto the shoots initiated higher responses in SOD, APX and GPX than did application to the soil surface, while the opposite was true for GR. (C) 1997 Elsevier Science Ltd. All rights reserved.