Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs
Transcriptional Reprogramming of Rice Cells by Xanthomonas oryzae TALEs
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DOI：
10.3389/fpls.2019.00162
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发表时间：
2019-02-25
影响因子：
5.6
通讯作者：
Boch, Jens
中科院分区：
文献类型：
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作者：
Muecke, Stefanie;Reschke, Maik;Boch, Jens
水稻致病性白叶枯病菌（Xanthomonas oryzae）引起严重的收成损失，并对稳定的粮食供应构成挑战。病原菌的毒力主要依赖于细菌TALE（transcription activator-like effector，转录激活子样效应子）蛋白，TALE蛋白在植物细胞内起转录激活子的作用。为了了解TALE的植物靶标，我们测定了印度X. oryzaepv.米（Xoo）型菌株ICMP 3125（T）和来自菲律宾的菌株PXO 142。分析了它们的完整TALE库，并表征了水稻中全基因组TALE靶点。结合计算靶标预测和水稻转录组学数据，我们能够验证12个特异性诱导的水稻靶标基因。在无TALE的Xoo菌株中重组并表达Xoo菌株的TALE，以将特异性诱导基因归因于单个TALE。使用报告基因分析，我们可以显示单个TALE直接作用于它们的靶启动子。特别地，我们表明，由AnnoTALE指定的TALE分类反映了共同的靶基因，并且Xoo和相关病原体X的TALE分类。oryzaepv.稻生菌具有比以前认为更多的共同靶基因。总之，我们建立了一个详细的图片TALE诱导的植物过程，显着扩大了我们的理解X。并将促进新抗性的发展以克服这一重要的水稻病害。
Rice-pathogenic Xanthomonas oryzae bacteria cause severe harvest loss and challenge a stable food supply. The pathogen virulence relies strongly on bacterial TALE (transcription activator-like effector) proteins that function as transcriptional activators inside the plant cell. To understand the plant targets of TALEs, we determined the genome sequences of the Indian X. oryzae pv. oryzae (Xoo) type strain ICMP 3125(T) and the strain PXO142 from the Philippines. Their complete TALE repertoire was analyzed and genome-wide TALE targets in rice were characterized. Integrating computational target predictions and rice transcriptomics data, we were able to verify 12 specifically induced target rice genes. The TALEs of the Xoo strains were reconstructed and expressed in a TALE-free Xoo strain to attribute specific induced genes to individual TALEs. Using reporter assays, we could show that individual TALEs act directly on their target promoters. In particular, we show that TALE classes assigned by AnnoTALE reflect common target genes, and that TALE classes of Xoo and the related pathogen X. oryzae pv. oryzicola share more common target genes than previously believed. Taken together, we establish a detailed picture of TALE-induced plant processes that significantly expands our understanding of X. oryzae virulence strategies and will facilitate the development of novel resistances to overcome this important rice disease.