p53 Protein overexpression in colorectal tumors from patients with familial adenomatous polyposis: is it an early or late event?
p53 Protein overexpression in colorectal tumors from patients with familial adenomatous polyposis: is it an early or late event?
复制标题
家族性腺瘤性息肉病患者结直肠肿瘤中 p53 蛋白过度表达：是早期事件还是晚期事件？
DOI：
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发表时间：
1996
影响因子：
9.8
通讯作者：
M. Rao
中科院分区：
文献类型：
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作者：
E. Levi;S. Stryker;M. Rao
目标
肿瘤发展是一个多步骤的过程，与多种遗传改变有关。家族性腺瘤性息肉病（FAP）是研究结直肠肿瘤发生中遗传学改变的经典范例。在这项研究中，我们通过免疫组化研究了FAP患者和散发性腺瘤和腺癌中p53过度表达的时间。
方法
我们检查了40个微腺瘤，114个管状腺瘤，3个腺癌，来自5个FAP患者和30个散发性腺瘤和14个散发性腺癌。
结果
在114例有轻度和中度异型增生的腺瘤中有43例观察到p53过表达，在3例腺癌中有3例观察到p53过表达，而在40例FAP患者的微腺瘤中没有观察到p53过表达。在散发性肿瘤中，30例中重度异型增生腺瘤中有6例，14例癌中有11例显示p53过表达。FAP患者、对照组患者和散发性肿瘤患者未受累的结肠粘膜不进行p53染色。
结论
这些结果表明，p53过度表达发生在早期的发展结直肠腺瘤FAP，而它是一个晚期事件的发展散发性肿瘤。
OBJECTIVES
Tumor development is a multistep process associated with multiple genetic alterations. Familial adenomatous polyposis (FAP) is a classical paradigm to study genetic alterations in the development of colorectal neoplasms. In this study, we investigated the timing of p53 overexpression by immunohistochemistry in colorectal carcinogenesis in FAP patients and in sporadic adenomas and adenocarcinomas.
METHODS
We examined 40 microadenomas, 114 tubular adenomas, and three adenocarcinomas from five FAP patients and 30 sporadic adenomas and 14 sporadic adenocarcinomas.
RESULTS
p53 overexpression was observed in 43 of 114 adenomas with mild and moderate dysplasia and in three of three adenocarcinomas and in none of 40 microadenomas from FAP patients. In sporadic tumors, six of 30 adenomas with moderate to severe dysplasia and 11 of 14 carcinomas showed p53 overexpression. Uninvolved colonic mucosa in FAP patients, control patients, and patients with sporadic tumors did not stain for p53.
CONCLUSIONS
These results indicate that p53 overexpression occurs early in the development of colorectal adenomas in FAP, whereas it is a late event in the development of sporadic tumors.