Effect of pre-ischaemic conditioning on hypoxic depolarization of dopamine efflux in the rat caudate brain slice measured in real-time with fast cyclic voltammetry
Effect of pre-ischaemic conditioning on hypoxic depolarization of dopamine efflux in the rat caudate brain slice measured in real-time with fast cyclic voltammetry
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DOI：
10.1016/j.neuint.2011.06.020
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发表时间：
2011-10-01
影响因子：
4.2
通讯作者：
Young, Andrew M. J.
中科院分区：
文献类型：
--
作者：
Davidson, Colin;Coomber, Ben;Young, Andrew M. J.
快速循环伏安法可用于测定缺氧缺糖（OGD）诱导的多巴胺释放。在这里，我们测量多巴胺流出与1秒的时间分辨率，这是适当的测量OGD诱发的多巴胺流出准确。在本研究中，我们研究了OGD诱发的多巴胺流出是否可以用来显示大鼠尾状脑切片缺血前的条件反射。尾状核切片暴露于0、2或10分钟OGD缺血前条件，然后60分钟后暴露于持续时间为15分钟的第二次OGD事件。我们使用快速循环伏安法测量OGD诱发的多巴胺流出，并且在一些实验中使用HPLC测量尾状体多巴胺和DOPAC组织水平，并且将20 μ m低温恒温器切片进行尼氏染色以指示神经元损失。我们发现，10分钟而不是2分钟的OGD缺血前预处理导致OGD诱发的多巴胺流出在主要OGD事件上的发作时间更长（10分钟与0分钟缺血前预处理分别为475 +/- 31和287 +/- 30 s）。此外，10分钟OGD预缺血处理导致第二次OGD事件时多巴胺流出减少（缺血前10分钟与缺血前0分钟分别为4.23 ± 1.12和8.14 ± 0.82 μ M），尽管这些切片具有相似的组织多巴胺含量和DOPAC/DA比率，在主要OGD事件中，多巴胺释放速率较慢（缺血前10分钟和缺血前0分钟分别为21 +/- 5和74 +/-8 nM/s）。这些数据表明，10分钟OGD缺血前的条件可以引起耐受的第二OGD事件和伏安记录的OGD诱发的多巴胺流出是一个有用的模型缺血前的条件在神经元组织。(C)2011 Elsevier B. V.保留所有权利。
Fast cyclic voltammetry can be used to measure dopamine release after oxygen and glucose deprivation (OGD) induced anoxic depolarization in vitro. Here we measure dopamine efflux with 1 s time resolution, which is appropriate to measure OGD-evoked dopamine efflux accurately. In the present study, we examined whether OGD-evoked dopamine efflux could be used to show pre-ischaemic conditioning in the rat caudate brain slice. Caudate slices were exposed to 0, 2, or 10 min OGD pre-ischaemic conditioning, then 60 min later exposed to a second OGD event of 15 min duration. We measured the OGD-evoked dopamine efflux using fast cyclic voltammetry and in some experiments caudate dopamine and DOPAC tissue levels were measured using HPLC and 20 mu m cryostat sections were Nissl stained to indicate neuronal loss. We found that 10 but not 2 min OGD pre-ischaemic conditioning resulted in a longer time to onset of OGD-evoked dopamine efflux on the main OGD event (475 +/- 31 and 287 +/- 30 s for 10 Vs 0 min pre-ischaemic conditioning respectively). Further, 10 min OGD pre-ischaemic conditioning resulted in less dopamine efflux on the second OGD event (4.23 +/- 1.12 and 8.14 +/- 0.82 mu M for 10 Vs 0 min pre-ischaemic conditioning respectively), despite these slices having similar tissue dopamine content and DOPAC/DA ratio, and the rate of dopamine release was slower in the main OGD event (21 +/- 5 and 74 +/- 8nM/s for 10 Vs 0 min pre-ischaemic conditioning respectively). These data suggest that 10 min OGD pre-ischaemic conditioning can evoke tolerance to a second OGD event and that voltammetric recording of OGD-evoked dopamine efflux is a useful model of pre-ischaemic conditioning in neuronal tissue. (C) 2011 Elsevier B.V. All rights reserved.