Arbuscular mycorrhizal fungi diversity in three different steppes of Xilingol, Inner Mongolia, China
Arbuscular mycorrhizal fungi diversity in three different steppes of Xilingol, Inner Mongolia, China
复制标题
内蒙古锡林郭勒盟三个不同草原丛枝菌根真菌多样性
作者：
Ren;jianting;Bao;yuying;Wang;qi;Xu;daolong;Li;xiufang
大多数陆生植物与丛枝菌根真菌（AMF）形成共生，这在几个方面使它们受益。不同丛枝菌根真菌群落诱导植物不同的生长反应，在决定生态系统变异性和生产力方面发挥着潜在的作用。以锡林郭勒盟冷蒿（Artemisia frigida）、大针茅（Stipa grandis）和羊草（Leymus chinensis）3种典型草原为研究对象，对其土壤理化性质、AMF定殖和AMF孢子进行了研究。从每个草地的草样品中扩增nu-clear小亚基rRNA基因来表征AMF群落。我们的研究结果表明，同一种植物表现出不同的AMF定殖在不同的草原。共鉴定出6属13种丛枝菌根真菌，其中Funneliformis constrictum、F.地孢囊霉和微团球囊霉是3个草原所有植物的优势种。孢子密度受植物种类和草原系统的显著影响。系统发育分析表明，筛选出的16个AMF序列具有较高的相似性，可能属于球囊霉科。本研究揭示了草地生态系统AMF群落水平的生物多样性，为草地生态系统健康评价和保护提供了理论依据。
The majority of terrestrial plants form symbioses with arbuscular mycorrhizal fungi (AMF) that benefit them in several ways..Different AMF communities induce different growth responses in plants playing a potential role to determine ecosystem variabil-ity and productivity. In this study, soil properties, AMF colonization and AMF spores were investigated in three typical steppes, namely, Artemisia frigida steppe, Stipa grandis steppe and Leymus chinensis steppe of Xilingol, Inner Mongolia, China. The nu-clear small subunit rRNA gene was amplified from grass samples from each grassland to characterize the AMF community. Our results demonstrated that the same plant displayed diverse AMF colonization in different steppes. Thirteen AMF species belong-ing to six genera were identified, among which Funneliformis constrictum, F. geosporus and Glomus microaggregatum were dominant species in all plants from three steppes. Spore density was significantly influenced by the plants species as well as the steppe system. Phylogenetic analysis demonstrated that the 16 screened AMF sequences showing high similarity may belong to the Glomeraceae. The present study reveals the grassland ecosystems biodiversity at the AMF community level, which provides theoretical basis for health evaluation and grassland ecosystem protection.