Inhibition of hepatitis B virus replication by quercetin in human hepatoma cell lines
Inhibition of hepatitis B virus replication by quercetin in human hepatoma cell lines
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DOI：
10.1007/s12250-015-3584-5
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发表时间：
2015-06-01
影响因子：
5.5
通讯作者：
Hu, Kanghong
中科院分区：
文献类型：
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作者：
Cheng, Zhikui;Sun, Ge;Hu, Kanghong
B型肝炎病毒（Hepatitis B virus，HBV）感染是世界上最严重和流行的病毒性疾病之一。虽然几种抗HBV药物已在临床上使用，但它们的副作用和不良反应限制了治疗效果。因此，有必要确定新的潜在的抗HBV药物。黄酮醇槲皮素已显示出对某些逆转录病毒的活性，但其对HBV的作用尚不清楚。在本研究中，槲皮素与HepG2.2.15细胞以及用HBV质粒转染的HuH-7细胞一起孵育。在两种细胞系中，槲皮素均显示出显著降低B型肝炎表面抗原（HBsAg）和B型肝炎e抗原（HBeAg）、分泌和HBV基因组DNA水平。此外，与拉米夫定（3 TC）、恩替卡韦（ETV）或阿德福韦（Ade）共孵育进一步增强了槲皮素诱导的HBV复制抑制。这种抑制部分与热休克蛋白和HBV转录水平降低有关。结果表明，槲皮素抑制HBV抗原分泌和基因组复制的人肝癌细胞株，这表明槲皮素可能是一个潜在的有效的抗HBV药物。
Hepatitis B virus (HBV) infection is one of the most serious and prevalent viral diseases in the world. Although several anti-HBV drugs have been used clinically, their side and adverse effects limit treatment efficacy. Therefore, it is necessary to identify novel potential anti-HBV agents. The flavonol quercetin has shown activity against some retroviruses, but its effect on HBV remains unclear. In the present study, quercetin was incubated with HepG2.2.15 cells, as well as HuH-7 cells transfected with an HBV plasmid. Quercetin was shown to significantly reduce Hepatitis B surface antigen (HBsAg) and Hepatitis B e antigen (HBeAg), secretion and HBV genomic DNA levels in both cell lines. In addition, co-incubation with lamivudine (3TC), entecavir (ETV), or adefovir (Ade) further enhanced the quercetin-induced inhibition of HBV replication. This inhibition was partially associated with decreased heat shock proteins and HBV transcription levels. The results indicate that quercetin inhibited HBV antigen secretion and genome replication in human hepatoma cell lines, which suggests that quercetin may be a potentially effective anti-HBV agent.