A POINT MUTATION IN THE LAST INTRON RESPONSIBLE FOR INCREASED EXPRESSION AND TRANSFORMING ACTIVITY OF THE C-HA-RAS ONCOGENE
A POINT MUTATION IN THE LAST INTRON RESPONSIBLE FOR INCREASED EXPRESSION AND TRANSFORMING ACTIVITY OF THE C-HA-RAS ONCOGENE
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DOI：
10.1038/334119a0
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发表时间：
1988-07-14
期刊：
影响因子：
64.8
通讯作者：
LEVINSON, AD
中科院分区：
文献类型：
--
作者：
COHEN, JB;LEVINSON, AD
Ha-ras原癌基因的T24/EJ等位基因具有强大的致癌活性，这不仅是因为有充分证据证明的突变扰乱了编码多肽的结构，而且还因为内含子中的第二个单核苷酸改变导致表达增加10倍。这种对表达的影响在周围DNA转移到异源基因后得以维持，因此定义了一种新的调控元件。
The T24/EJ allele of the Ha-ras proto-oncogene owes its powerful oncogenic activity not merely to the well documented mutation that perturbs the structure of the encoded polypeptide, but in addition to a second single nucleotide alteration in an intron that causes a tenfold increase in expression. This effect on expression is maintained upon transfer of the surrounding DNA to a heterologous gene, and as such defines a novel regulatory element.