Architecture and Characteristics of Bacterial Nanotubes
Architecture and Characteristics of Bacterial Nanotubes
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DOI：
10.1016/j.devcel.2016.01.013
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发表时间：
2016-02-22
影响因子：
11.8
通讯作者：
Ben-Yehuda, Sigal
中科院分区：
文献类型：
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作者：
Dubey, Gyanendra P.;Mohan, Ganesh Babu Malli;Ben-Yehuda, Sigal
细菌展示出一系列依赖于接触的相互作用系统，这些系统已经进化到促进细胞间的直接通信。我们之前已经确定了一种由桥接相邻细胞的纳米管介导的细菌通讯模式。在这里，我们阐明了纳米管结构、动力学和分子成分。利用枯草芽孢杆菌作为模型生物，我们发现在低细胞密度下，纳米管表现出显着的复杂性，既以细胞间管又以延伸管的形式存在，后者经常以“根状”的方式围绕细胞。实时观察纳米管的形成表明，这些结构在几分钟内形成，并表现出快速的运动。利用超分辨率、光学和电子显微镜的结合，我们揭示了纳米管是由具有此外，我们发现纳米管中存在一种保守的钙调磷酸酶样蛋白 YmdB，并且是纳米管生产和细胞间分子贸易所必需的。
Bacteria display an array of contact-dependent interaction systems that have evolved to facilitate direct cell-to-cell communication. We have previously identified a mode of bacterial communication mediated by nanotubes bridging neighboring cells. Here, we elucidate nanotube architecture, dynamics, and molecular components. Utilizing Bacillus subtilis as a model organism, we found that at low cell density, nanotubes exhibit remarkable complexity, existing as both intercellular tubes and extending tubes, with the latter frequently surrounding the cells in a "root-like'' fashion. Observing nanotube formation in real time showed that these structures are formed in the course of minutes, displaying rapid movements. Utilizing a combination of super-resolution, light, and electron microscopy, we revealed that nanotubes are composed of chains of membranous segments harboring a continuous lumen. Furthermore, we discovered that a conserved calcineurin-like protein, YmdB, presents in nanotubes and is required for both nanotube production and intercellular molecular trade.